Immunoproteomic analysis of secretory proteins ofAspergillus fumigatus with specific IGE immunoreactivity |
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| Authors: | Poonam Gautam Taruna Madan W N Gade P Usha Sarma |
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| Institution: | (1) Institute of Genomics and Integrative Biology, Deihi;(2) Department of Biotechnology, University of Pune, Pune;(3) Department of Plant Pathology, Indian Agricultural Research Institute, Pusa, Delhi |
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| Abstract: | Allergenic/antigenic proteins are known to induce Type I and Type III hypersensitivity reactions leading to allergic bronchopulmonary
aspergillosis (ABPA) in immunocompetent host. The common structural features or intrinsic properties of the allergens/antigens
leading to allergenicity in a host are not well understood. In the current report, comparative analysis of proteins on two
dimensional gel electrophoresis (2D-3) and specific IgE immunoblots ofA. fumigatus secretory proteins (1st, 2nd and 3rd week culture filtrate proteins) was carried out. We observed a total of 159 proteins in 1st, 2nd and 3rd week culture filtrates ofA. fumigatus. Specific IgE immunoreactivity was observed in 75 proteins with different intensity. Third week culture filtrate showed maximum
number of proteins, 142, and specific IgE immunoreactive proteins, 65. MALDI-TOF analysis resulted in putative identification
of two allergens as hypothetical protein YBL057c fromSaccharomyces cereviseae and unnamed protein product fromDebaryomyces hansenii (similar to IPF14568 ofCandida albicans). Identification of a repertoire of specific IgE immunoreactive proteins will facilitate the studies on structure-function
relationship of these proteins relevant for diagnosis and pathogenesis. |
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| Keywords: | Immunoproteomics Aspergillus fumigatus |
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