| 氨基酸分离分析方法与衰老和疾病关系的研究 |
| |
| 作者姓名： | 杨莉丽 袁倬斌 孙汉文 |
| |
| 作者单位： | 1. 中国科学院研究生院化学教学部, 北京 100039;
2. 河北大学化学系, 保定 071002 |
| |
| 摘 要： | 为了检验对映体的纯度, 考察对映体的生物活性, 必须发展与之相对应的简便 、快速、准确、灵敏的分离和测定方法.目前,对映体的分离方法主要有非色谱法和色谱法两大类.常用的非色谱分离法主要包括化学拆分法、膜拆分法和电化学法等等,但是近年来在氨基酸对映体拆分中应用较少.1952 年Delgiesh 采用纸层析分离氨基酸对映体, 并提出“三点作用”分离机理, 揭开了色谱技术分离手性氨基酸的序幕.
|
| 收稿时间： | 2001-04-25 |
Study on Separation and Analysis of Amino Acids andRelationships Between Amino Acidsand Senescence and Diseases |
| |
| Authors: | Yang Lili Yuan Zhuobin Sun Hanwen |
| |
| Institution: | 1. The Graduate School of the Chinese Academy of Sciences, Beijing 100039;
2. Heber Umversity, Baoding 071002 |
| |
| Abstract: | Capillary electrophoresis(CE)has become a popular separate and analytical technique through the development for 20 years since it was introduced in the early of 1980s.With the combination of traditional elec-trophoresis and modern high performance liquid chromatography(HPLC), CE has been widely used in life science,biochemistry, environmental science, medical science and pharmaceutical chemistry with the advantageous aspects of higher separation effciency, smaller sample volume, lower operation cost and stronger resistance to interferences.The separation of enantiomers,which demands high sensitivity, highselectivity and high separation efficiency, is main challenge to chemistry, especially analytical chemistry.Chromatography techniques, including HPLC, thin layer chromatography(TLC), gas chromatography(GC)and high performance capillary electrophoresis(HPCE), are com-monly used for the chiral separation.However, the disadvantages of long analytical time, expensive chiral column,complicated manipulation and the large volume of solvent waste have limited the application o HPLC,which is the most universal chiral separation method.The rapid developing CE technique has become an attractive alternative for the chiral separation and has been applied to many resarch fields.Amino acids are the fundamental units of protein which is the main component of the living organisms.The sep-aration and analysis of biological moleculars, including amino acid, polypeptide, DNA, nucleic acid and protein, are attractive and difficult topics in analytical chemistry and life science.Therefore, it has momentous academic and clin-ical practice significance to develop simple, fsat, accurate, sensitive methods for esparation and analysis of aminoacids and investigate the relationships between amino acids and senescence, diabetes, liver cancer and coronary dis-eases.The major contents of this dissertation are described in the following chapter. |
| |
| Keywords: | |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|
