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| 成龄栓皮栎组培苗生根影响因素研究 | |
| 引用本文： | 杨锋利,杜保国,张存旭.成龄栓皮栎组培苗生根影响因素研究[J].绵阳师范学院学报,2006,25(5):79-82,95. |
| 作者姓名： | 杨锋利 杜保国 张存旭 |
| 作者单位： | 1. 绵阳师范学院生命科学与工程系,四川,绵阳,621000;西北农林科技大学林学院,陕西,杨凌,712100 2. 绵阳师范学院生命科学与工程系,四川,绵阳,621000 3. 西北农林科技大学林学院,陕西,杨凌,712100 |
| 基金项目： | 绵阳师范学院青年教师科研基金项目(MA2005021) |
| 摘 要： | 以成龄(30年以上)栓皮栎枝条的萌条在室内沙培催芽,用萌芽作为外植体建立无菌苗体系,研究了基本培养基、蔗糖浓度以及IBA浓度和处理时间对生根的影响。结果表明:采用1/4MS培养基添加30g/L蔗糖为较适宜的培养基,生根率达到了66.4%,平均生根数为2.3条,平均根长为4.82 cm。采用两步生根法,用添加1.0mg/L的IBA培养基处理14天,然后转接到无激素的培养基上的生根率最高,达到了96.7%,愈伤组织少。 |
| 关 键 词： | 栓皮栎 组培 生根 影响因素 |
| 文章编号： | 1672-612x（2006）05-0079-04 |
| 收稿时间： | 04 23 2006 12:00AM |
| 修稿时间： | 2006-04-23 |
A Study of the Factors Influencing the Rooting of the Tissue Cultural Seedlings of Mature Quercus Variabilis |
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| YANG Feng-li,DU Bao-guo,ZHANG Cun-xu.A Study of the Factors Influencing the Rooting of the Tissue Cultural Seedlings of Mature Quercus Variabilis[J].Journal of Mianyang Normal University,2006,25(5):79-82,95. | |
| Authors: | YANG Feng-li DU Bao-guo ZHANG Cun-xu |
| Institution: | 1 College of Life Science and Engineering,Mianyang Normal University, Mianyang, Sichuan,621000;2 College of Forestry, Northwest Sci -Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100 |
| Abstract: | Accelerating the germination of branches obtained from over 30 years old quercus variabilis trees by indoor sand culture, then using coppice sprouts from the branches as explants to build tissue cultural system, this experiment studies the effect of the cultural media kinds,the sucrose concentration,the IBA concentration and the processing time on the ratio of rooting. The result shows that 1/4MS with 30g/L sucrose is the best medium, the rooting ratio is up to 66.4%, the average number of the rooting is 2.3, and the average length of the roots is 4. 82cm; following the two -step rooting method, the shoots have been cultured by adding 1.0mg/L IBA for 14 days, then transferred to the medium without hormone. In this way, a high rooting ratio is up to 96.7%, and there is no callus in the base. |
| Keywords: | quercus variabilis tissue culture rooting influencing factors |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |