| 持续被动运动通过抑制软骨细胞NO合成下调MMP-1和MMP-13表达 |
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| 引用本文： | 李洪武.持续被动运动通过抑制软骨细胞NO合成下调MMP-1和MMP-13表达[J].广州体育学院学报,2015,35(3):90-95. |
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| 作者姓名： | 李洪武 |
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| 作者单位： | 郑州航空工业管理学院体育部,河南郑州,450015 |
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| 摘 要： | 目的:观察持续被动运动(CPM)和补充一氧化氮(NO)供体——硝酸甘油(NG)对兔骨关节炎(OA)模型中软骨基质金属蛋白酶-1(MMP-1)和MMP-13表达以及NO含量的影响,探讨CPM下调MMPs的可能机制。方法:30只3~4月龄雄性新西兰大白兔,其中6只进行假手术作为正常对照组(NC组),另外24只建立膝关节OA模型,术后随机分为4组,即OA对照组(OA组)、OA硝酸甘油给药组(NG组)、OA持续被动运动组(CPM组)、OA持续被动运动+硝酸甘油给药组(CPM+NG组),每组各6只。NC组和OA组不作处理,NG组给予NG软膏膝关节局部涂抹,CMP组在CPM训练仪上进行膝关节持续被动运动,CMP+NG组即在运动干预的同时给予NG局部涂抹。4周后取各组软骨组织,硝酸还原酶法测定NO含量,HE染色观察软骨形态学变化并进行Mankin’s评分,实时荧光定量PCR(RQ-PCR)检测MMP-1和MMP-13 mRNA水平,免疫组织化学法测定MMP-1和MMP-13蛋白表达量。结果:NO含量、Mankin’s评分以及MMP-1和MMP-13 mRNA和蛋白水平在OA组明显高于NC组(P<0.01),NG组高于OA组(P<0.01),CPM组低于OA组和NG组(P<0.01),CPM+NG组低于NG组(P<0.01),但高于CPM组(P<0.01)。结论:CPM通过抑制软骨细胞NO合成下调MMP-1和MMP-13表达,进而对软骨细胞起保护作用。
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| 关 键 词： | 持续被动运动 骨关节炎 软骨 一氧化氮 基质金属蛋白酶 |
| 修稿时间： | 2015/1/6 0:00:00 |
Continuous Passive Motion Downregulated Expression of MMP-1 and MMP-13 Via Inhibition of NO Synthesis in Chondrocytes |
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| LI,Hong-wu.Continuous Passive Motion Downregulated Expression of MMP-1 and MMP-13 Via Inhibition of NO Synthesis in Chondrocytes[J].Journal of Guangzhou Physical Education Institute,2015,35(3):90-95. |
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| Authors: | LI Hong-wu |
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| Institution: | LI Hong-wu;Dept. of PE,Zhengzhou Institute of Aeronautical Industry Management; |
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| Abstract: | Objective: To observe the effects of continuous passive motion (CPM) and nitric oxide (NO) donor- nitroglycerin (NG) supplement on matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-13 (MMP-13) expression and NO content in cartilage of rabbit osteoarthritis (OA) model and investigate the possible mechanism that CPM downregulated MMPs. Methods: Of thirty male New Zealand white rabbits of 3-4 months, six sham operated served as normal control group (NC, not treated) and the other twenty-four animals being created OA model were randomly allocated four group: osteoarthritis control group (OA, not treated), nitroglycerin group (NG, treated with NG ointment on knee joint), continuous passive motion group (CPM, exercise on CPM training apparatus) and continuous passive motion+nitroglycerin group (CPM+NG, exercise plus NG supplement). After four weeks, NO content were measured by nitrate reductase method, cartilage morphologic variations by HE stain and Mankin''s score, MMP-1 and MMP-13 mRNA by real-time fluorescent quantitation PCR (RQ-PCR) and the protein level by immunohistochemistry. Results: NO content, Mankin''s score, MMP-1 and MMP-13 mRNA and protein level in OA group were higher than those in NC group (P<0.01) and in NG group higher than OA group, while the indexes above in CPM group were lower than OA and NG group and in CPM+NG group lower than NG group but higher than CPM group. Conclusion: CPM downregulated expression of MMP-1 and MMP-13 via inhibition of NO synthesis in chondrocytes, thus protecting chondrocytes in OA model. |
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| Keywords: | continuous passive motion osteoarthritis cartilage nitric oxide matrix metalloproteinase |
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