| 不同强度运动对大鼠骨骼肌蛋白合成信号影响的研究 |
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| 引用本文： | 赵华,曾凡星,王宁琦.不同强度运动对大鼠骨骼肌蛋白合成信号影响的研究[J].北京体育大学学报,2014,37(12):45-50+71. |
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| 作者姓名： | 赵华 曾凡星 王宁琦 |
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| 作者单位： | 北京体育大学运动人体科学学院，北京 100084；华中师范大学体育学院，湖北 武汉 430079;北京体育大学运动人体科学学院，北京 100084;北京体育大学运动人体科学学院，北京 100084 |
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| 基金项目： | 国家自然科学基金项目：“雄激素对运动骨骼肌 mTOR 信号的调控及机理”（项目编号：31071034）。通信作者：赵华。 |
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| 摘 要： | 摘要：目的：本研究旨在研究不同强度的急性运动对骨骼肌蛋白合成信号的影响，以期能深入阐明运动对骨骼肌蛋白合成代谢的调控机理。方法：8周龄SD雄鼠分别进行不同强度的跑台运动，于运动后即刻、6h 和12h 取材白腓肠肌。BCA法测肌肉蛋白浓度，Western Blot法测肌肉MHC和AR、mTOR、p70S6K、4EBP1、MEK、ERK和p90RSK的磷酸化。结果：①骨骼肌AR磷酸化在中强度运动后即刻、恢复期6h和12h分别增加13%、20%和14%，而在高强度运动后分别增加16%、57%和37%；②mTOR磷酸化在中强度和高强度运动后3个时间点均显著增加，分别增加19%、37%、4%和28%、53%、4%；p70S6K磷酸化分别增加28%、76%、18%和33%、96%、25%。4EBP1磷酸化分别增加18%、33%、7%和25%、44%、5%；③MEK磷酸化在在中强度和高强度运动后3个时间点均显著增加，分别增加74%、22%、3%和106%、51%、24%。ERK磷酸化分别增加39%、29%、11%和55%、32%、22%。p90RSK磷酸化分别增加22%、16%、4%和35%、20%、6%。结论：①骨骼肌AR活性、mTOR通路和MAPK通路的活性均存在运动强度依赖性，前两者均于运动后恢复期6h达到最高值，后者于运动后即刻达到最高值。②在运动后的恢复期中，AR活性在mTOR通路和MAPK通路大幅回落之后，仍保持较高的活性。
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| 关 键 词： | 关键词：适应性肥大 运动 雄激素受体（AR） 哺乳动物雷帕霉素靶蛋白（mTOR） 丝裂原活化蛋白激酶（MAPK） |
| 收稿时间： | 2014/6/23 0:00:00 |
Effects of Different Intensity Exercise on Protein Synthesis of Skeletal Muscle in Rats |
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| ZHAO Hu,ZENG Fan-xing and WANG Ning-qi.Effects of Different Intensity Exercise on Protein Synthesis of Skeletal Muscle in Rats[J].Journal of Beijing Sport University,2014,37(12):45-50+71. |
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| Authors: | ZHAO Hu ZENG Fan-xing and WANG Ning-qi |
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| Institution: | Sport Science School, Beijing Sport University, Beijing 100084, China; College of Physical Education, Central China Normal University, Wuhan 430079, Hubei China;Sport Science School, Beijing Sport University, Beijing 100084, China;;Sport Science School, Beijing Sport University, Beijing 100084, China; |
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| Abstract: | Abstract: Objective: The purposes of this study were to examine effects of different intensity exercise on protein synthesis of skeletal muscle, and to further illustrate the regulation mechanism of exercise on skeletal muscle protein metabolism. Methods: Eight-week old male Sprague-Dawley rats were divided into two groups to undergo different intensity exercise. Gastrocnemius was isolated at time point of immediately, 6h and 12h after exercise. The protein concentration of skeletal muscle was determined by BCA assay. The protein expression of MHC and the phosphorylation expression of AR, mTOR, p70S6K, 4EBP1, MEK, ERK and p90RSK of skeletal muscle were examined by Western blotting. Results: 1) Phosphorylation of AR had increased significantly after moderate (immediately: 13%; 6h: 20%; 12h: 14%) and high intensity exercise (immediately: 16%; 6h: 57%; 12h: 37%) respectively. 2) Phosphorylation of mTOR had increased significantly after moderate (immediately: 19%; 6h: 37%; 12h: 4%) and high intensity exercise (immediately: 28%; 6h: 53%; 12h: 4%) respectively. Phosphorylation of p70S6K had increased significantly (moderate intensity: immediately: 28%, 6h: 76%, 12h: 18%; high intensity: immediately: 33%, 6h: 96%, 12h: 25%). Phosphorylation of 4EBP1(Thr37/46) had increased significantly (moderate intensity: immediately18%, 6h: 33%, 12h: 7%; high intensity: immediately: 25%, 6h: 44%, 12h: 5%). 3) Phosphorylation of MEK1/2 had increased significantly after moderate (immediately: 74%; 6h: 22%; 12h: 3%) and high intensity exercise (immediately: 106%; 6h: 51%; 12h: 24%). Phosphorylation of ERK1/2 had increased significantly (moderate intensity: immediately: 39%, 6h: 29%, 12h: 11%; high intensity: immediately: 55%, 6h: 32%, 12h: 22%). Phosphorylation of p90RSK had increased significantly (moderate intensity: immediately: 22%, 6h: 16%, 12h: 4%; high intensity: immediately: 35%, 6h: 20%, 12h: 6%). Conclusions: 1) AR bioactivity, mTOR pathway and MAPK pathway are dependent on exercise intensity. The first two reached the peak at 6h after exercise, and the last one was at immediately after exercise. 2) AR still has high activity after the sharp drop of mTOR pathway and MAPK pathway during exercise recovery. |
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| Keywords: | Key words: adaptive hypertrophy exercise androgen receptor (AR) mammalian target of rapamycin (mTOR) mitogen-activated protein kinase (MAPK) |
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