| 不同强度力竭运动大鼠运动后12h肝细胞凋亡的研究 |
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| 引用本文： | 陈伟,樊新生,张廷妍,李善妮.不同强度力竭运动大鼠运动后12h肝细胞凋亡的研究[J].北京体育大学学报,2010(2). |
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| 作者姓名： | 陈伟 樊新生 张廷妍 李善妮 |
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| 作者单位： | 泰山学院体育科学系;中南大学应用生物科学技术系; |
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| 摘 要： | 研究对象、方法:利用细胞免疫组化的方法研究力竭运动后12 h成年雄性SD大鼠肝细胞凋亡同肝细胞内的自由基水平和钙代谢之间的关系。结果:1)运动组与C组相比,凋亡细胞的数量差异显著(p<0.05);运动组组间差异也具有显著意义(p<0.05)。2)ME、HE组肝细胞内SOD活性与C组相比差异显著(p<0.05),但运动组组间差异不显著。3)ME组肝细胞中MDA含量增加、GSH含量降低,且相对于对照组差异显著(p<0.05);HE组虽有类似变化,但与其它各组相比无显著性差异。4)运动组肝细胞中Ca2+含量较对照组增加,差异显著(ME:p<0.01,HE:p<0.05),运动组组间差异显著(p<0.05)。结论:1)力竭运动可导致肝细胞凋亡,氧化应激及钙离子超载是引起细胞凋亡的重要因素,且前者可能发挥更重要作用。2)中等强度力竭运动更易于导致肝细胞凋亡,其原因可能同长时间力竭运动引起中暑或严重缺水,进而导致恢复期内中性粒细胞发生呼吸爆发有关。
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| 关 键 词： | 肝细胞凋亡 氧化自由基 线粒体Ca2+ |
A Study on the Hepatic Cells Apoptosis in Rats after 12 Hours ofCessation of Different Intensity Exhaustive Exercise |
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| CHEN Wei,FAN Xin-sheng,ZHANG Ting-yan,LI Shan-ni.A Study on the Hepatic Cells Apoptosis in Rats after 12 Hours ofCessation of Different Intensity Exhaustive Exercise[J].Journal of Beijing Sport University,2010(2). |
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| Authors: | CHEN Wei FAN Xin-sheng ZHANG Ting-yan LI Shan-ni |
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| Institution: | 1.Department of Sport Medicine;Taishan Medicine University;Taian 271021;Shandong China;2.School of Biological Science and Technology;Central China University;Changsha 410083;Hunan China |
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| Abstract: | With the method of immunohistochemica,the paper attempts to explore the correlation among hepatic cell apoptosis,level of oxygen free radicals and mitochondria calcium in ischemic/reperfusion hepatic cells of the male mouse.As a result,it is found that:1)in HE group and ME group,there are manydetectable apoptotic hepatic cells,which are significant compared with that in the control group.In addition,the difference of apoptotic cells between two exercise groups is also significant(p<0.05).2) The SOD activiti... |
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| Keywords: | hepatic cell apoptosis oxygen free radicals mitochondrial calcium |
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