| 跑台运动对大鼠骨骼肌mTOR mRNA 和蛋白表达的影响 |
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| 引用本文： | 贺道远,曾凡星,翟昕元.跑台运动对大鼠骨骼肌mTOR mRNA 和蛋白表达的影响[J].天津体育学院学报,2008,23(5):386-389. |
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| 作者姓名： | 贺道远 曾凡星 翟昕元 |
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| 作者单位： | 1. 三峡大学体育学院,宜昌,443002
2. 北京体育大学,北京,100084 |
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| 基金项目： | 国家自然科学基金，三峡大学校科研和教改项目 |
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| 摘 要： | 目的:mTOR信号调控骨骼肌蛋白翻译过程,本研究旨在探讨耐力运动对骨骼肌mTOR蛋白表达的影响.方法:SD大鼠分为运动组和对照组.运动组大鼠进行跑台坡度5°,上坡跑,速度20m/min,60min/次的跑台运动后12 h,采用RT-PCR方法测定腓肠肌mTOR mRNA,westerm blot测定mTOR蛋白的表达.结果:1次急性运动后大鼠骨骼肌mTOR mRNA和蛋白表达显著上升(P<0.05),4周持续耐力运动后大鼠骨骼肌mTOR mRNA和蛋白表达显著上升(P<0.05).结论:耐力运动后骨骼肌mTOR表达上升,提示mTOR可能参与肌肉生长对运动适应的调控.
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| 关 键 词： | 耐力运动 翻译调控 哺乳动物雷帕霉素靶蛋白（mTOR） |
Effects of Treadmill Exercise on mTOR mRNA and Protein Expression of Skeletal Muscle in Rats |
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| HE Daoyuan,ZENG Fanxing,ZHAI Xinyuan.Effects of Treadmill Exercise on mTOR mRNA and Protein Expression of Skeletal Muscle in Rats[J].Journal of Tianjin Institute of Physical Education,2008,23(5):386-389. |
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| Authors: | HE Daoyuan ZENG Fanxing ZHAI Xinyuan |
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| Institution: | School of PE, Three Gorges University, Hubei 443002, China;;Beijing Sports University, Beijing 100084, China);School of PE, Three Gorges University, Hubei 443002, China; |
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| Abstract: | Aim: The mTOR signal regulates protein translation of skeletal muscle. The purpose was to investigate the effect of endurance exercise on the expression of mTOR protein in skeletal muscle. Methods: SD rats were divided into control group and exercise group. Using RT- PCR and western blot, the mTOR mRNA and protein expression of gastrocnemius muscle in rats were measured in 12 hours after treadmill exercise with 5°incline, 20m/min, 60min/ time. Results: The mTOR mRNA and protein expression increased significantly in exercise groups (p<0.05) after one time acute exercise and so did the 4 weeks endurance exercise. Conclusions: The mTOR expression increased after endurance exercise. The data suggested that mTOR may be involved in the regulation of adaptation of muscle growth to exercise. |
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| Keywords: | endurance exercise translation regulation mammalian target of Rapamycin (mTOR) |
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