| 补充活性肽对大鼠运动后骨骼肌中间丝蛋白免疫染色的影响 |
| |
| 引用本文： | 李世成,焦海舟,吴维,刘军.补充活性肽对大鼠运动后骨骼肌中间丝蛋白免疫染色的影响[J].天津体育学院学报,2006,21(2):135-138. |
| |
| 作者姓名： | 李世成 焦海舟 吴维 刘军 |
| |
| 作者单位： | 1. 湛江师范学院,体育科学学院,湛江,524048
2. 解放军422医院,湛江,524005 |
| |
| 摘 要： | 目的:观察补充活性肽对大鼠骨骼肌细胞结蛋白和波形蛋白免疫染色和血清酶活性的影响,研究活性肽对运动性骨骼肌微结构损伤的保护作用。方法:雄性SD大鼠100只,依分别补充安慰剂和活性肽,运动与不运动,以及运动后0h、12h、24h和48h等不同时间随机分为10组,所有动物膳食平衡1周后,进行实验。运动组大鼠以(20±1)m/min的速度,坡度为-16,°持续性跑台训练120min。实验组和对照组所有动物在膳食平衡期间每天分别进行灌胃15%活性肽饮料2mL和等量的安慰剂。结果:补充活性肽可减轻大鼠离心运动后骨骼肌结蛋白免疫染色的丢失,增强波形蛋白的免疫染色,明显减少血清酶CK、LDH的漏出。
|
| 关 键 词： | 活性肽 骨骼肌 中间丝蛋白 免疫染色 离心运动 大鼠 |
| 文章编号： | 1005-0000（2006）02-0135-05 |
| 收稿时间： | 2005-12-25 |
| 修稿时间： | 2005-12-252006-02-20 |
Effects of Soy Hydrolyzed Peptides Supplementation on Immunostaining of Intermediate Filaments
of Skeletal Muscle After a Exercise of Rats |
| |
| LI Shi-cheng,JIAO Hai-zhou,WU Wei,LIU Jun.Effects of Soy Hydrolyzed Peptides Supplementation on Immunostaining of Intermediate Filaments
of Skeletal Muscle After a Exercise of Rats[J].Journal of Tianjin Institute of Physical Education,2006,21(2):135-138. |
| |
| Authors: | LI Shi-cheng JIAO Hai-zhou WU Wei LIU Jun |
| |
| Institution: | Dept. of Sports Sciences, Zhanjiang Normal Institute, Zhanjiang 524048, China;Dept. of Sports Sciences, Zhanjiang Normal Institute, Zhanjiang 524049, China;No. 422 Hospital of PLA, Zhanjiang 524005, China;No. 422 Hospital of PLA, Zhanjiang 524006, China |
| |
| Abstract: | Objective: Through immunochemistry methods, the protection effects of soy hydrolyzed peptides supplementation on micro- injury of skeletal muscle
after intensive training were examined. Methods: 100 Male Sprague- Dawley rats were randomly divided into 10 groups including placebo, SHPs supplemented,
exercise or not, ohr, 12hr, 24hr and 48hr after exercise. Rats in exercise group did 120 minutes exercise at speed of 20±1m/min in running platform
with - 16°. Animals in control and experimental groups were fed 2ml beverage containing 15% SHPs and same placebo respectively. Results: Supplementation
of SHPs alleviated obviously the loss of immunostaining for desmin, increased the immunostaining for vimentin following exercise, and decreased
obviously the concentration of blood serum enzyme CK, LDH. |
| |
| Keywords: | soy hydrolyzed peptides skeletal muscle intermediate filaments immunostaining eccentric exercise rat |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《天津体育学院学报》浏览原始摘要信息 |
| 点击此处可从《天津体育学院学报》下载免费的PDF全文 |
|
