| 不同模式低氧训练对急性力竭运动下大鼠肝脏、肾脏线粒体呼吸链复合体酶活性的影响 |
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| 引用本文： | 李洁,吴刚,王世超.不同模式低氧训练对急性力竭运动下大鼠肝脏、肾脏线粒体呼吸链复合体酶活性的影响[J].天津体育学院学报,2011,26(2):128-130. |
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| 作者姓名： | 李洁 吴刚 王世超 |
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| 作者单位： | 西北师范大学体育学院,甘肃兰州,730070 |
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| 基金项目： | 国家自然科学基金项目（项目编号：31060145） |
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| 摘 要： | 经5周不同模式低氧训练,测定急性力竭运动即刻大鼠肝脏、肾脏线粒体呼吸链酶复合体Ⅰ、Ⅱ和Ⅲ的活性,探讨不同低氧训练模式对肝脏及肾脏线粒体呼吸链功能的影响。方法:40只2月龄雄性Wistar大鼠随机均分为5组:常氧训练组(LoLo)、高住高练组(HiHi)、高住低训组(HiLo)、低住高练组(LoHi)和高住高练低训组(HiHiLo)。各组大鼠分别在常氧(海拔1 500 m,大气压632 mmHg)或/和低氧(海拔3 500 m,大气压493 mmHg)环境中居住及递增负荷训练5周。力竭运动后即刻取样。差速离心法提取线粒体。分光光度法测定呼吸链酶复合体Ⅰ、Ⅱ和Ⅲ活性。结果显示:HiLo模式和HiHiLo模式可显著提高大鼠力竭运动后即刻肝脏线粒体呼吸链功能,4种低氧训练模式均降低了大鼠力竭运动后即刻肾脏线粒体呼吸链功能,不同低氧训练模式对大鼠力竭运动后即刻线粒体呼吸链功能的影响存在组织差异性。
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| 关 键 词： | 低氧训练 肝脏 肾脏 线粒体 呼吸链 酶复合体 |
Effect of Different Hypoxia Training Modes on RCC Activities in Rat Liver and Kidney after Exhaustive
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| LI Jie,WU Gang,WANG Shichao.Effect of Different Hypoxia Training Modes on RCC Activities in Rat Liver and Kidney after Exhaustive
Running[J].Journal of Tianjin Institute of Physical Education,2011,26(2):128-130. |
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| Authors: | LI Jie WU Gang WANG Shichao |
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| Institution: | (School of PE,Northwest Normal University,Lanzhou 730070,China) |
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| Abstract: | The aim of this paper is to study the effect of hypoxic training on activities of respiratory chain complexes in mitochondria of liver and kidney in rats.40 healthy male Wister rats were randomized to 5 group(n=5):living low-training low(LoLo),living high-training high(HiHi),living high-training low(HiLo),living low-training high(LoHi) and living high-exercise high-training low(HiHiLo).All the animals performed five weeks’ training in normoxic(Atmospheric pressure=632 mmHg,altitude of about 1 500 m) or hypoxic environment(Atmospheric pressure=493 mmHg,simulated altitude of about 3 500 m).After exhaustive running,instantly takes a sample.Mitochondria were extracted by the method of different centrifugation.Spectrophotometric analysis was used to evaluate RCC(Ⅰ-Ⅲ) activities in liver and kidney mitochondria.The results suggested that HiLo and HiHiLo could significantly improve activities of respiratory chain function in mitochondria from liver.Different methods of hypoxic training could decrease activities of respiratory chain function in mitochondria from kidney. |
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| Keywords: | hypoxic training liver kidney mitochondria respiratory chain multi-enzymatic |
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