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| 不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究 | |
| 引用本文： | 李洁,杨静,赵丽丽.不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究[J].武汉体育学院学报,2019,53(5):88-94. |
| 作者姓名： | 李洁 杨静 赵丽丽 |
| 作者单位： | 1. 西北师范大学 体育学院, 甘肃 兰州 730070; 2. 甘肃民族师范学院, 甘肃 合作 747000 |
| 基金项目： | 国家自然科学基金项目（31760295）。 |
| 摘 要： | 目的:探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法:将雄性Wistar大鼠50只随机均分为5组:安静对照组(C)、低负荷运动训练组(LT)、中等负荷运动训练组(MT)、高负荷运动训练组(HT)和极高负荷运动训练组(ST),每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后,取腓肠肌样本,提取线粒体,测定线粒体柠檬酸合成酶(CS)、异柠檬酸脱氢酶(ICD)和α—酮戊二酸脱氢酶(α-KGDHC)活性;线粒体Ga2+含量、胞浆NADH、NAD^+、ATP和ADP含量,以及ICD mRNA转录水平。结果:(1)不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组(P<0.01),且CS和ICD活性由高到低顺序均为:MT组>HT组>ST组>LT组>C组,α-KGDHC活性由高到低顺序为:HT组>MT组>ST组>LT组>C组。(2)不同负荷运动训练组线粒体Ca2+含量均显著高于安静对照组(P<0.01),其含量由高到低顺序为:MT组>HT组>ST组>LT组>C组;胞浆NADH/NAD^+和ATP/ADP的比值均显著低于安静对照组(P<0.01),其比值由低到高顺序为:MT组 |
| 关 键 词： | 骨骼肌 运动负荷 三羧酸循环 酶活性 基因表达 Ca2+含量 NADH/NAD+ ATP/ADP |
| 收稿时间： | 2019-03-02 |
Effect of Different Load Exercise Training on Mitochondrial Tricarboxylic Acid Cycle in Rat Skeletal Muscle and its Mechanism |
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| LI Jie,YANG Jing,ZHAO Lili.Effect of Different Load Exercise Training on Mitochondrial Tricarboxylic Acid Cycle in Rat Skeletal Muscle and its Mechanism[J].Journal of Wuhan Institute of Physical Education,2019,53(5):88-94. | |
| Authors: | LI Jie YANG Jing ZHAO Lili |
| Institution: | 1. College of P. E., Northwest Normal Univ., Lanzhou 730070, China; 2. Gansu Normal Univ. for Nationalities, Hezuo 747000, China |
| Abstract: | The objective of this paper was to study of effect of different load exercise training on the mitochondrial tricarboxylic acid cycle in rat skeletal muscle and its mechanism. Fifty male Wistar rats were randomly divided into 5 groups(n=10):control (C),low-intensity exercise training (LT),mid-intensity exercise training (MT),high intensity exercise training (HT) and strong high intensity exercise training (ST) groups. LT,MT,HT and ST groups received 6-week treadmill training. After training of 6 weeks, all the rats from 5 groups were sacrificed to obtain gastrocnemius muscles samples, and muscle mitochondria were extracted by differential centrifugation. The activities of citrate synthase (CS), isocitrate dehydrogenase (ICD), α-ketoglutarate dehydrogenase (α-KGDHC), content of Calcium in mitochondria, content of NADH, NAD+, ATP and ADP in cytoplasm and ICD gene expression level were measured. The results were as follows:(1)In the different intensity exercise training group, the activity of CS、ICD and α-KGDHC were increased significantly compared with C group in gastrocnemius muscle of mitochondria (P<0.01). CS and ICD activity was MT > HT > ST > LT > C and α-KGDHC activity was followed by HT > MT > ST > LT > C. (2) In the different intensity exercise training group, the content of Ca2+ were increased significantly compared with C group in gastrocnemius muscle of mitochondria (P < 0.01). Its contentwas followed as MT > HT > ST > LT > C; NADH/NAD + and ATP/ADP ratio were increased significantly compared with C group in rat gastrocnemius muscles of mitochondria(P<0.01), and the ratio was followed as MT < ST < HT < LT < C.(3)In the different intensity exercise training group,ICD expression level were increased significantly by compared with C group in mitochondria of rat gastrocnemius muscles (P<0.01),and the expression level was followed as MT > HT > ST > LT > C. It could be concluded that the low-intensity, mid-intensity,high intensity and strong high intensity exercise training could increase activities of limiting enzymes of tricarboxylic acid cycle in skeletal muscle mitochondria. Mid-high intensity exercise training had the best effect. Its mechanism was related to NADH/NAD+ and ATP/ADP in cytoplasm, ability of mitochondria calcium uptake and enzymatic gene expression level. |
| Keywords: | skeletal muscle exercise load tricarboxylic acid cycle enzymatic activity gene expression calcium content NADH/NAD+ ATP/ADP |
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