| 不同游泳运动对增龄大鼠肾组织内皮素-1及其受体的影响 |
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| 引用本文： | 章岚,邢文华,陈勇,张一民.不同游泳运动对增龄大鼠肾组织内皮素-1及其受体的影响[J].体育科学,2004,24(8):30-34,49. |
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| 作者姓名： | 章岚 邢文华 陈勇 张一民 |
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| 作者单位： | 1. 山东体育学院,基础理论系,山东,济南,250063
2. 北京体育大学,研究生院,北京,100084 |
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| 基金项目： | 20 0 1年度博士点基金资助 (2 0 0 10 0 43 0 0 1 |
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| 摘 要： | 探讨了不同持续时间和运动频率的游泳运动对增龄大鼠肾脏 ET- 1及其受体的影响。研究对象为 2月龄和 9～ 10月龄雄性 SD大鼠 ,按体重将它们随机分成 8组 ,分别为安静对照组、运动 1h(6次 /周 )组、运动 2 h(6次 /周 )组和运动 1h(3次 /周 )组。运动组进行 14周的无负重游泳训练。采用放免法测定肾组织 ET- 1含量 ;EL ISA法测定肾组织 ETA- R浓度 ;黄嘌呤氧化酶法测定肾组织 SOD活性 ,硫代巴比妥酸 (TBA)比色法测定 MDA含量 ,考马斯亮兰法测定蛋白含量。结果 :1)随着增龄 ,肾组织 ET- 1含量明显升高 (P<0 .0 1) ;ETA - R浓度则降低 (P<0 .0 1) ;SOD/ MDA比值的变化没有显著性。 2 )运动后 ,6月龄大鼠肾组织 ET- 1含量与对照组的差异没有显著性 ;而 13～ 14月龄大鼠 ET- 1含量明显下降 (P<0 .0 1) ,而且表现为每周运动频率较高组下降幅度较大。 3)运动后 ,原 2月龄大鼠肾组织 ETA- R浓度的变化均没有显著性。结论 :1)随着增龄 ,肾组织 ET- 1含量明显增加 ,而 ETA- R浓度明显降低 ;2 )当增龄诱导的 ET- 1浓度升高时 ,运动可以降低其含量 ,运动对 ET- 1的作用表现为每周运动频率较高组降低幅度较大。 3)运动对肾组织 ETA- R的浓度没有明显的影响。 4 ) SOD/ MDA比值与 ET- 1和 ETA- R的分泌没有明显的因果关
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| 关 键 词： | 内皮素-1 动物实验 鼠 游泳 影响 |
| 文章编号： | 1000-677X(2004)08-0030-05 |
Effects of Different Swimming Training on Endothelin-1 and Endothelin-1 Receptor in Renal Tissue in Aged Rats |
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| ZHANG Lan,XING Wen-hua,CHEN Yong,ZHANG Yi-min.Depantment of Basic Theories,Shandong Physical Education Institute,Jinan ,China,.Graduate School,Beijing Sports University,Beijing ,China..Effects of Different Swimming Training on Endothelin-1 and Endothelin-1 Receptor in Renal Tissue in Aged Rats[J].China Sport Science,2004,24(8):30-34,49. |
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| Authors: | ZHANG Lan XING Wen-hua CHEN Yong ZHANG Yi-minDepantment of Basic Theories Shandong Physical Education Institute Jinan China Graduate School Beijing Sports University Beijing China |
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| Institution: | ZHANG Lan1,XING Wen-hua1,CHEN Yong2,ZHANG Yi-min11.Depantment of Basic Theories,Shandong Physical Education Institute,Jinan 250063,China,2.Graduate School,Beijing Sports University,Beijing 100084,China. |
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| Abstract: | The purpose of this paper is to investigate the effect of different duration and frequency of swimming on endothelin-1 and Endothelin-1 receptor in renal tissue in aged rats.The subjects are 2 and 9~10 month old rats divided into 8 groups randomly,that is sedentary control group,one hour exercise group (six days per week),two hour exercise group (six days per week),0ne hour exercise (three days per week).ET-1 protein in renal tissue is determined by radioimmunoassay.The concentration of ETA-R in renal tissue is measured by ELISA.The level of SOD,MDA is determined.The result shows that with the age increasing,the concentration of ET-1 in renal tissues increases significantly ( P<0.01 ),but that of ETA-R reduces significantly,and SOD/MDA has no change significantly.Comparing with control group,the concentration of ET-1 in renal tissue has no change significantly for six months swimming training rats,but reduces significantly in 13~14 month rats ( P<0.01 ).ET-1 decreases lower in swimming 6 days per week than 3 days per week.Swimming training does not affect the concentration of ETR-R in renal tissue.With the increasing of age,the concentration of ET-1 in renal tissue increase significantly,while that of ETA-R reduces significantly.Swimming training can reduce renal ET-1 level.Swimming training does not have any effect on ETA-R level.There is no cause-effect relation among ET-1,ETA-R level and SOD/MDA. |
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| Keywords: | endothelin-1 animal experiment rat swim age influence |
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