| 大强度运动对大鼠心肌线粒体膜通透性转换孔状态的影响 |
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| 引用本文： | 苏全生,段意梅,李顺昌,孙涛,孙君志.大强度运动对大鼠心肌线粒体膜通透性转换孔状态的影响[J].体育科学,2007,27(11):48-54. |
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| 作者姓名： | 苏全生 段意梅 李顺昌 孙涛 孙君志 |
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| 作者单位： | 1. 成都体育学院,四川,成都,610041
2. 大理学院,云南,大理,671003 |
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| 摘 要： | 目的观察大鼠一次性大强度游泳运动后不同时相心肌线粒体膜通透性转换孔(PTP孔)的性状和心肌超微结构等指标的变化情况,探讨线粒体损伤与PTP孔变化之间的关系.方法将32只成年雄性SD大鼠随机分为A组(空白对照组,n=8)和B组(大强度运动组,n=24).B组又随机分为运动后即刻组(B1组,n=8),运动后12 h组(B2组,n=8)和运动后24 h组(B3组,n=8).检测各组大鼠线粒体前向角散射(FSC)、线粒体90°侧向角散射(SSC)和线粒体内Rhodamine123的荧光强度值(FL1);电镜观察各组大鼠左室前壁心肌纤维超微结构变化情况.结果B2组FSC值显著高于其他3组(P＜0.05),B2组SSC值和FL1值显著低于其他3组(P＜0.05).电镜结果表明,大强度运动可造成大鼠心肌线粒体损伤,以运动后12 h损伤最为严重.结论大强度游泳运动可能诱发大鼠心肌线粒体膜通透性转换孔的开放,促使线粒体外的物质内流并影响线粒体内结构的改变;大面积的线粒体损伤可能与心肌线粒体膜通透性转换孔的开放有重要的关联性.
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| 关 键 词： | 鼠 大强度运动 流式细胞仪 心肌线粒体 线粒体膜通透性转换孔 游泳 动物实验 |
| 文章编号： | 1000-677X（2007）11-0048-07 |
| 收稿时间： | 2007-09-26 |
| 修稿时间： | 2007-10-15 |
Effects of High Intensive Exercise on Myocardial Mitochondrial Membrane Permeability Transition Pore of Rats |
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| SU Quan-sheng,DUAN Yi-mei,LI Shun-chang,SUN Tao,SUN Jun-zhi.Effects of High Intensive Exercise on Myocardial Mitochondrial Membrane Permeability Transition Pore of Rats[J].China Sport Science,2007,27(11):48-54. |
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| Authors: | SU Quan-sheng DUAN Yi-mei LI Shun-chang SUN Tao SUN Jun-zhi |
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| Abstract: | The purpose of this study was to observe the mitochondrial membrane permeability transition pore and ultrastructure of rats in different phase after high intensive swimming,explore the relationship between exercise and mitochondrial membrane permeability transition pore.Thirty-two SD rats (male) were randomly divided into normal control group (group A,n=8) and high intensive exercise group (group B,n=24).Group B were divided into B1,2,B3 (N=8) respectively according to the instant,12h and 24h after exercise.The changes in FSC,Rhodamine 123 fluorescence and SSC of the mitochondrial were determined by ELITE-ESP flow cytometer.The myocardial fiber changes of anterior of left ventricle were observed under Transmission Electron Microscope (TEM).The result shows the FSC of group B2 was significant higher than other groups (P<0.05),the SSC and FL1 of group B2 was significant lower than other groups (P<0.05).High intensive exercise may cause myocardial mitochondrial damage.It concludes that high intensive exercise may induce PTP to open.It also hints that there is certain relationship between the large area of mitochondrial injury and the opening of mitochondrial membrane permeability transition pore. |
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| Keywords: | rats high intensive ezercise flow c ytometer myocardial mitochondrial membrane permeability transition pore |
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