| 长期运动训练对外周血淋巴细胞DNA损伤和凋亡的影响及机制研究 |
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| 引用本文： | 代志军,朱荣.长期运动训练对外周血淋巴细胞DNA损伤和凋亡的影响及机制研究[J].山东体育学院学报,2012,28(3):60-65. |
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| 作者姓名： | 代志军 朱荣 |
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| 作者单位： | 1. 潍坊学院,山东潍坊,261061
2. 温州医学院,浙江温州,325035 |
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| 基金项目： | 浙江省教育厅科研计划项目 |
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| 摘 要： | 训练组(男子足球运动员,n=16)和对照组(男子大学生,n=16)在功率自行车上完成一次递增负荷力竭运动。运动前、运动后以及运动后24 h采集静脉血,测定外周血淋巴细胞计数、淋巴细胞凋亡率、羟自由基、8-羟基脱氧鸟嘌呤(8-OHdG)和8-氧鸟嘌呤DNA糖基化酶(OGG1)。结果发现,运动后即刻,训练组和对照组外周血淋巴细胞计数(P<0.01;P<0.05)、淋巴细胞凋亡率(P<0.05;P<0.01)、羟自由基(P<0.01;P<0.01)、8-OHdG(P<0.01;P<0.01)和OGG1(P<0.01;P<0.01)均高于运动前水平,而训练组OGG1升高幅度高于对照组(P<0.01),其他各指标升高幅度则均明显低于对照组(均为P<0.01)。运动后24h,对照组外周血淋巴细胞计数低于运动前(P<0.01),淋巴细胞凋亡率高于运动前(P<0.01),而训练组则恢复至运动前;两组8-OHdG(P<0.05;P<0.01)均高于运动前,但升高幅度明显低于对照组(P<0.01);OGG1在对照组恢复至运动前,而训练组仍高于运动前水平(P<0.05)。因此,长期运动训练通过降低氧化应激水平、提高DNA修复能力、减轻DNA氧化损伤,抑制运动性淋巴细胞凋亡。
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| 关 键 词： | 运动训练 外周血淋巴细胞 DNA损伤 细胞凋亡 DNA修复酶 |
Effects and mechanism of long - term exercise training on peripheral blood lymphocyte DNA damage and apoptosis |
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| DAI Zhi-jun
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ZHU Rong.Effects and mechanism of long - term exercise training on peripheral blood lymphocyte DNA damage and apoptosis[J].Journal of Shandong Physical Education Institute,2012,28(3):60-65. |
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| Authors: | DAI Zhi-jun ZHU Rong |
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| Institution: | 1.Weifang University,Weifang 261061,China;2.Wenzhou Medical College,Whnezhou 325035,China |
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| Abstract: | Training group(male football athletes,n=16) and control group(male normal undergraduate students,n=16) performed a bout of incremental exhausted exercise test on cycle ergometer.Peripheral blood lymphocyte counts,apoptotic rate of leukocytes,hydroxy radical,8-hydroxydeoxyguanosine(8-OHdG) and 8-oxoguanine DNA glycosylase(OGG1) were determined in venous blood before,immediately after and 24 h after exercise test.We found that although lymphocyte count(P<0.01;P<0.05),apoptotic rate(P<0.05;P<0.01),hydroxyl radical(P<0.01;P<0.01),8-OHdG(P<0.01;P<0.01) and OGG1(P<0.01;P<0.01) increased immediately after exercise test in both groups,the change extent of OGG1 in training group was significantly higher than that of control group(P<0.01),while that of other indexes were lower than control group(all P<0.01).After 24h,lymphocyte count was lower(P<0.01),apoptotic rate was higher(P<0.01) than that of pre-exercise in control group,which were recovered in training group;8-OHdG in both group were higher(P<0.05;P<0.01)than pre-exercise but elevation range in training group was lower than control group(P<0.01);OGG1 recovered in control group,while higher than pre-exercise in training group(P<0.05).In conclusion,attenuated oxidative stress,improved DNA repair ability and decreased lymphocyte oxidative DNA damage could be the mechanism to prevent exercise induced lymphocytes apoptosis through long-term exercise training. |
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| Keywords: | exercise training peripheral blood lymphocyte DNA damage apoptosis DNA repair enzyme |
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