| 游泳池水氯化消毒对游泳训练效果影响的组织病理学与代谢组学研究 |
| |
| 引用本文： | 李江华,王智慧,朱小娟,葛耀军.游泳池水氯化消毒对游泳训练效果影响的组织病理学与代谢组学研究[J].中国体育科技,2012,48(3):141-145. |
| |
| 作者姓名： | 李江华 王智慧 朱小娟 葛耀军 |
| |
| 作者单位： | 江西师范大学,江西南昌,330022 |
| |
| 基金项目： | 江西省教育科学“十二五”规划课题(11YB161) |
| |
| 摘 要： | 目的:了解传统氯化消毒方式产生的副产物对游泳训练效果的影响,并利用组织病理学和基于核磁共振(NMR)的代谢组学方法分析这种影响产生的原因及其生理、生化机制.方法:SD雄性大鼠12只,随机分为对照组与实验组各6只,进行为期10周的渐增负荷游泳训练.实验组游泳池水按照普通游泳池水质标准投入次氯酸钙氯化消毒；对照组游泳池水为纯净水.10周训练结束后,首先收集尿样,做代谢组学分析；然后进行负重耐力游泳能力测试,记录大鼠游泳至力竭的时间；测试完成后,大鼠休息2天(48 h),断头处死,取肺进行组织病理切片.结果:实验组大鼠游泳至力竭的时间为28.97±2.55 min,明显低于对照组的39.15±9.85 min(P＜0.05)；大部分大鼠肺泡壁出现了毛细血管扩张充血,肺泡腔有水肿液,但是未见器质性病变,实验组和对照组检测结果基本一致,没有明显差异；实验组大鼠代谢表型(1H NMR代谢指纹图谱)与对照组相比出现了明显的、有群体性特征的变化,其中主要代谢标志物为:γ-氨基丁酸、丙酮酸和马尿酸.结论:按标准剂量对游泳池水投放含氯消毒剂进行氯化消毒没有引起受训大鼠肺部出现器质性病变,但是对耐力游泳训练效果有一定的不良影响,氯化消毒副产物诱发的肝脏解毒功能亢奋和机体代谢表型的变化可能是这一影响产生的主要原因.
|
| 关 键 词： | 代谢组学 氯化消毒 核磁共振 模式识别 游泳 鼠 动物实验 |
Histopathology and Metabolomics Study on the Influence of Chlorinated Pool Water on Swimming Training Effects |
| |
| LI Jiang-hua
,
WANG Zhi-hui
,
ZHU Xiao-juan
,
GE Yao-jun.Histopathology and Metabolomics Study on the Influence of Chlorinated Pool Water on Swimming Training Effects[J].China Sport Science and Technology,2012,48(3):141-145. |
| |
| Authors: | LI Jiang-hua WANG Zhi-hui ZHU Xiao-juan GE Yao-jun |
| |
| Institution: | Jiangxi Normal University,Nanchang 330022,China. |
| |
| Abstract: | Objective: To explore the influence of the chlorinated pool water to swimming training effects and analyze the biological mechanism which makes this influence happen.Method:Twelve male SD rats were randomly separated into experimental group and control group,an increasing load swimming training was carried out for ten weeks.The pool water of the experimental group was disinfected with calcium hypochlorite,while the pool water used for the control group was purified with a water purifier.After ten weeks training,the urine samples were collected for metabolomics analysis,and then a swimming capacity test was taken by recording the swimming time to exhaustion of each rat.Two days(48 h) later,all rats were decapitated and their lungs were collected for histopathology analysis.Results:The exhaustion time of experimental group was 28.97±2.55 minutes,which is significantly lower than the control group’s 39.15±9.85 minutes(P<0.05);No significant differences were found between two groups in the histopathology analysis.There were no organic disease appearing,but the capillary vessels around alveolus of both groups expanded and congested.Compared to the control group,the metabolic phenotype of experimental group significantly changed,and the main metabolic biomarkers were hippurate,pyruvate,γ-amino-n-butyrate.Conclusion:Chlorination of the swimming pool water with standard dose doesn’t induce organic disease to the trained rats,but it does impair the training effects.Liver detoxification function excitement and the changed metabolic phenotype,induced by disinfection byproducts,might be the main factors for this influence. |
| |
| Keywords: | metabolomics chlorination NMR pattern recognition swimming rat animal experiment |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
