| 基因表达谱芯片筛选甲状腺癌失分化相关基因的初步研究 |
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| 引用本文： | 李志刚,邓敬兰,汪静,侯英萍.基因表达谱芯片筛选甲状腺癌失分化相关基因的初步研究[J].少年体育训练,2006(3). |
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| 作者姓名： | 李志刚 邓敬兰 汪静 侯英萍 |
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| 作者单位： | 第四军医大学西京医院核医学科 陕西西安710032 |
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| 基金项目： | 国家自然科学基金资助(项目编号30270413) |
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| 摘 要： | 目的应用基因芯片技术筛查甲状腺癌失分化相关基因,为进一步研究其发病机制提供新的思路和线索。方法分别用Cy5和Cy3两种不同的荧光染料通过逆转录反应将失分化甲状腺癌组和对照组分化型甲状腺癌组织的mRNA分别标记成探针,并与载有一组靶基因的基因表达谱芯片进行杂交。通过扫描荧光强度,计算机软件分析,寻找两组差异表达基因。结果分化型甲状腺癌与失分化甲状腺癌组织之间存在一系列差异表达基因,共有373条差异表达基因,其中表达增加的有209条(2.0倍以上),表达降低的有164条(0.5倍以下)。结论基因芯片为筛选甲状腺癌失分化相关基因提供了有效的方法。
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| 关 键 词： | 基因芯片 甲状腺癌 失分化 相关基因 |
Screening of differentially expressed genes related to dedifferentiated thyroid cancer by cDNA microarray. |
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| Abstract: | Objective To screen the differentially expressed genes between differentiated thyroid cancer and dedifferentiated thyroid cancer tissues using cDNA microarray,and identify new targets and strategies for the pathogenesis of dedifferentiated thyroid cancer. Methods mRNA from both differentiated thyroid cancer and dedifferentiated thyroid cancer tissues were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP(Cy-5 or Cy-3) to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray, which was scanned for the fluorescent signals and showed difference between two tissues. Results A series of differentially expressed genes were found between two tissues. 209 up-regulated and 164 down-regulated genes were identified among the 373 differentially expressed genes. Conclusion cDNA microarry provides a promising approach to specific gene expressions of thyroid cancer dedifferentiated. |
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| Keywords: | cDNA microarray thyroid cancer dedifferentiated gene related |
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