| 浙江省12个主栽茭白品种亲缘关系的RAPD分析 |
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| 引用本文： | 俞晓平,张珏锋,吕仲贤,陈建明,郑许松,徐红星,陈列忠.浙江省12个主栽茭白品种亲缘关系的RAPD分析[J].科技通报,2005,21(2):137-141. |
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| 作者姓名： | 俞晓平 张珏锋 吕仲贤 陈建明 郑许松 徐红星 陈列忠 |
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| 作者单位： | 1. 中国计量学院,生命科学院,杭州,310018;浙江省农业科学院,植物保护与微生物研究所,杭州,310021
2. 浙江省农业科学院,植物保护与微生物研究所,杭州,310021 |
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| 基金项目： | 浙江省自然科学基金青年科技人才专项(RC97018)
浙江省重点科技攻关项目(2004C22004)
浙江省自然科学基金(M303411)资助 |
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| 摘 要： | 采用RAPD技术构建了12个浙江主栽茭白品种的RAPD指纹图谱。利用河姆渡双季茭作为模板从50个随机引物中筛选出稳定且多态性高的7个引物用于茭白DNA的PCR扩增,共产生45条DNA片段,平均每个引物的扩增带为6.4条,其中15条扩增带具有遗传多样性。约占总DNA片段的33.3％。利用相似性聚类分析对PCR扩增产物进行二态性数据分析,结果表明,在相似系数2.1处可以将12个茭白品种分为5个聚类群,其中9个浙江本地品种间的遗传距离较近。初步结果也表明,单季茭与双季茭间的遗传差异比同一类型中茭白品种间的差异显著。
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| 关 键 词： | 遗传育种 茭白品种 RAPD 遗传距离 浙江 |
| 文章编号： | 1001-7119(2005)02-0137-05 |
| 修稿时间： | 2004年2月14日 |
DNA Polymorphism of 12 Selected Water Oat Varieties (Zizania caduciflora) Collected from Zhejiang |
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| YU Xiao-ping,ZHANG Jue-Feng,LU Zhong-Xian,CHEN Jian-ming,ZHENG Xu-Song,XU Hong-xing,CHEN Lie-Zhong.DNA Polymorphism of 12 Selected Water Oat Varieties (Zizania caduciflora) Collected from Zhejiang[J].Bulletin of Science and Technology,2005,21(2):137-141. |
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| Authors: | YU Xiao-ping ZHANG Jue-Feng LU Zhong-Xian CHEN Jian-ming ZHENG Xu-Song XU Hong-xing CHEN Lie-Zhong |
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| Abstract: | 7 primers were screened from 50 arbitrary primers to study the DNA polymorphism of 12 varieties of Water oat (Z. caduciftora) collected in Zhejiang province. Totally 45 DNA fragments were generated, and the average number of DNA band produced by each primer was 6.4, 15 of them varied between varieties, occupied about 33.3%. Nei's similarity coefficients and genetic distances between varieties were calculated, and the clustering analysis of amplifying DNA bands showed that 12 varieties were divided into 5 clustering groups and 9 landrace collected from Zhejiang province had a close similarity. The genetic differences between single and double cropping varieties are larger than these between varieties in the same cropping type. |
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| Keywords: | genetic breeding Zizania caduciflora RAPD genetic distance Zhejiang |
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