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| 大鼠帕金森氏病动物模型的建立 | |
| 引用本文： | 郑杰胜,郑树森,詹仁雅,周永庆,许德华,张家兴.大鼠帕金森氏病动物模型的建立[J].科技通报,2001,17(6):80-83. |
| 作者姓名： | 郑杰胜 郑树森 詹仁雅 周永庆 许德华 张家兴 |
| 作者单位： | 1. 浙江大学医学院附属第一医院神经外科 2. 浙江大学医学院附属第一医院器官移植中心 3. 中国科学院上海生物化学研究所 4. 浙江大学医学院解剖教研室 |
| 摘 要： | 采用SD大鼠30只，25只为试验组，5只为对照组。在三维立体定向仪上用微量注射器将6－OHDA32μg分两点注射入右侧纹状体，每周检测动物的旋转行为，第1周动物没有出现旋转行为，第2周动物出现明显的旋转行为，随后缓慢上升；至术后5周才升至较高的水平并保持平稳。其中有15只动物达到或超过了7r/min这一通常采用的标准，模型成功率达60％。酪氨酸羟化酶免疫组化显示：术后第1周至第5周右侧黑质TH免疫反应细胞逐渐砬少，各个时期的右侧黑质TH免疫反应细胞较对侧明显减少。采用此方法建立的大鼠帕金森氏病模型更加符合临床PD病人的病程进展并明显提高成功率。 |
| 关 键 词： | 帕金森氏病 动物模型 6－OHDA 中枢神经系统变性疾病 大鼠 TH免疫反应细胞 |
| 文章编号： | 1001-7119(2001)06-80-04 |
| 修稿时间： | 2001年7月17日 |
Establishment of animal models of Parkinson′s disease |
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| ZHENG Jie sheng ,ZHENG Shu sen ,ZHAN Ren ya ,ZHOU Yong qing ,XU De hua ,ZHANG Jia xing.Establishment of animal models of Parkinson′s disease[J].Bulletin of Science and Technology,2001,17(6):80-83. | |
| Authors: | ZHENG Jie sheng ZHENG Shu sen ZHAN Ren ya ZHOU Yong qing XU De hua ZHANG Jia xing |
| Institution: | ZHENG Jie sheng 1,ZHENG Shu sen 2,ZHAN Ren ya 1,ZHOU Yong qing 1,XU De hua 3,ZHANG Jia xing 4 |
| Abstract: | Stereotaxic injection of 6 hydroxydopamine into the striatum of rats was performed to develop Parkinson`s disease models in thirty SD rats.One week after 6 OHDA injection into the striatum, no Apomorphine induced rotation was recorded, while obvious rotation appeared after two weeks, turns increased in the following 4 weeks, average 8.5 turns/min was recorded in fifth week and the rotation was stable in the following 1 month. The turns of 15 rats exceeded 7 turns/time.With the method of immunocytochemistry of Tyrosine hydroxylase(TH), the amount of TH immunoreactive(TH IR) neuronal cell numbers in the SN ipsilateral to the injection was decreased progressively,but no changes was seen in the right SN and in the control rats. These results suggested that the animal behavioral change after stereotaxic injection of 6 OHDA into striatum was much more like the progressive development of clinic PD patients. |
| Keywords: | parkinson`s disease animal model 6 OHDA |
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