| 脂肪酸合成酶缩合中心的羧负离子结合部位 |
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| 引用本文： | 田维熙.脂肪酸合成酶缩合中心的羧负离子结合部位[J].中国科学院研究生院学报,1990(2). |
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| 作者姓名： | 田维熙 |
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| 作者单位： | 中国科学院中国科学技术大学研究生院生物教学部 中国科学院生物物理研究所生物大分子开放实验室 |
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| 摘 要： | 本文对六个修饰巯基的试剂失活脂肪酸合成酶(FAS)的动力学进行了系统研究,发现它们与FAS的作用是否属于亲和标记,修饰巯基时的专一性,以及盐和NADPH有无保护,均只与该试剂分子中有无羧基负离子有关,提出在FAS的缩合中心存在一个对羧负离子有特异亲和力的结合部位.该部位可能是丙二酰基在缩合中心的结合部位,为缩合反应所必需,它使携带了丙二酰基的活臂向缩合中心运动,并结合于此进行缩合反应.
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| 关 键 词： | 脂肪酸合成酶 动力学 巯基修饰 缩合中心 |
THE LOADING SITE OF CARBOXYL ANION ON CONDENSATION CENTER OF FATTY ACID SYNTHETASE |
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| Tian Weixi.THE LOADING SITE OF CARBOXYL ANION ON CONDENSATION CENTER OF FATTY ACID SYNTHETASE[J].Journal of the Graduate School of the Chinese Academy of Sciences,1990(2). |
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| Authors: | Tian Weixi |
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| Abstract: | The inactivation kinetics of fatty acid synthctase(FAS) by modification of six SH reagents has been studied. It is found that the type of kinetics, the specificity of modification and protection by Salt or NADPH, are decided only by the carboxyl anion in reagent. It has been suggested that a carboxyl anion loading site exist in condensation center of FAS. This site is very likely the loading site of malonyl which contains a carboxyl anion, and is essential to condensation. |
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| Keywords: | fatty acid synthetasc kinetics modification of sulfhydryl group condensation center |
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