Effect of antimalarials treatment on rat liver lysosomal function—Anin vivo study |
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| Authors: | Samir P Patel Subhash D Katewa Surendra S Katyare |
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| Institution: | (1) Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, 390 002 Vadodara, India;(2) Department of Biological Sciences, The University of Iowa, 200 Biology Building, 52246 Iowa, USA |
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| Abstract: | Effects of treatmentin vivo with the antimalarials:chloroquine (CQ), primaquine (PQ) and quinine(Q) on lysosomal enzymes and lysosomal membrane integrity
were examined. Treatment with the three antimalarials showed an apparent increase in the membrane stability. CQ treatment
resulted in increase in both the ‘free’ and ‘total’ activities of all the enzymes i.e. acid phosphatase, RNase II, DNase II
and cathepsin D. PQ treatment lowered the ‘free’ and ‘total’ activities of acid phosphatase and cathepsin D, but the DNase
II activities increased. Treatment with Q resulted in increased ‘free’ and ‘total’ activities of RNase II and DNase II. While
‘free’ activities of acid phosphatase and cathepsin D were low; the ‘total’ activities increased significantly. Our results
suggest that a generalized increase in free nucleases activities following prolonged treatment with antimalarials may lead
to cell damage and/or necrosis. |
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| Keywords: | lysosomes lysosomal enzymes acid phosphatase RNase II DNase II cathepsin D T/F ratio |
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