| 酶法提取罗非鱼鱼鳍胶原蛋白的研究 |
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| 引用本文： | 王春,周天,方素云.酶法提取罗非鱼鱼鳍胶原蛋白的研究[J].茂名学院学报,2013(6):24-26. |
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| 作者姓名： | 王春 周天 方素云 |
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| 作者单位： | 广东石油化工学院化学与生命科学学院,广东茂名525000 |
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| 摘 要： | 文章对罗非鱼鱼鳍胶原蛋白的提取进行研究。通过单因素试验研究鱼鳍胶原蛋白提取的不同因素影响作用，并通过正交试验确定胃蛋白酶酶法提取胶原蛋白的最佳工艺。研究结果表明，在酶解时间6h、底物浓度5％、酶量5500U／g、pH为1．80和温度为40℃的条件下，胃蛋白酶水解罗非鱼鱼鳍效果较好。正交试验表明，酶法提取反应主要因素影响的顺序为：反应时间〉酶量〉底物浓度。
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| 关 键 词： | 罗非鱼鱼鳍 胃蛋白酶 胶原蛋白 |
On the Extraction of Collagen Protein from Tilapia Fin with Papain |
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| Institution: | WANG Chun, ZHOU Tian, FANG Su- yun (College of Chemistry and Life Science, Guangdong University of Petrochemical Technology, Maoming 525000, China) |
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| Abstract: | The extraction of collagen protein from tilapia fin is studied. The single factor experiment was conducted to study the effects of dif- ferent factors on pepsin hydrolysis of tilapia, and the best hydrolysis technology of tilapia pepsin hydrolysis were determined by orthogonal test. The results showed that the better effect of pepsin hydrolysis is in the enzymolysis time 6h, substrate concentration 5%, the amount of enzyme 5500 U/g, pH 1.80, the temperature 40 ~C. Orthogonal test showed that the order of effect is： reaction time 〉 enzyme amount 〉 substrate concentration. |
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| Keywords: | tilapia fin pepsin hydrolysis collagen protein |
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