A pair of two-component regulatory genes<Emphasis Type="Italic">ecr</Emphasis>A1/A2 in<Emphasis Type="Italic">S. coelicolor</Emphasis> |
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| Authors: | Email author" target="_blank">Li?Yong-quanEmail author Cen?Pei-lin Chen?Shi-fei Wu?Dan Zheng?Jing |
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| Institution: | (1) College of Life Science, Zhejiang University, 310027 Hongzhou, China;(2) Institute of Bioengineering, Zhejiang University, 310027 Hongzhou, China;(3) Drug Adiministration of Zhejiang Province, 310012 Hongzhou, China |
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| Abstract: | Two-component genes are kinds of genetic elements involved in regulation of antibiotic production inStreptomyces coelicolor. DNA microarray analysis revealed thatecrA1/A2, which mapped at distant sites fromred lucus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway.ecrA1 andecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed
that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the
actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed
that the two-component systemecrA1/A2 was positive regulatory element forred gene cluster.
Project (No.20172046) supported by the National Natural Foundation of China |
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| Keywords: | Streptomyces coelicolor Two-component system Antibiotic gene cluster ecrA1/A2 |
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