Study of cellulolytic soil fungi and two nova species and new medium |
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| Authors: | Mahmood Khalid Wei-jun Yang Nazir Kishwar Zahid Iqbal Rajput Abdullah G Arijo |
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| Institution: | (1) Government College, Satellite Town, Rawalpindi, 46000, Pakistan;(2) School of Life Sciences, Zhejiang University, Hangzhou, 310058, China;(3) Natural History Museum, Islamabad, 44000, Pakistan;(4) R & D Section, Directorate of Animal Husbandry Sindh, Shahbaz Building, Hyderabad, 71000, Pakistan;(5) Department of Parasitology, Sindh Agriculture University, Tando Jam, 70060, Pakistan |
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| Abstract: | This study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi.
The soil samples were inoculated into culture plates prepared in Sabouraud medium under sterilized conditions and incubated
at 30 °C for 4 to 7 d. The identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic
ability. Forty-two species, including 2 nova species, representing sixteen genera showed growth and sporulation in the cellulose
medium. Most of the isolated species were from genus Aspergillus and Penicillium. Aspergillus niger and Mucor hiemalis showed highest occurrence frequency (45% and 36% respectively), as these species were collected from about 80% of soil samples.
Being agar free and cheaper, the new fungal medium designed showed results equivalent to Sabouraud medium. |
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| Keywords: | Cellulolytic soil fungi Cellulose medium Nova species Taxonomy |
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