Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir |
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| Authors: | Patrick C Y Woo Samson S Y Wong Jade L L Teng Kit-Wah Leung Antonio H Y Ngan Dong-qing Zhao Herman Tse Susanna K P Lau and Kwok-Yung Yuen |
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| Institution: | (1) Division of Infectious Diseases, Duke University Medical Center, Box 3824, Durham, NC 27710, USA;(2) Durham VA Medical Center, Durham, NC, USA;(3) North Shore Hospital, Auckland, New Zealand;(4) Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand;(5) Duke Clinical Research Institute, Durham, NC, USA;(6) American University of Beirut Medical Center, Beirut, Lebanon;(7) Medical Center Ljublijana, Ljublijana, Slovenia;(8) Prince of Wales Hospital, Sydney, Australia;(9) King Faisal Hospital, Riyadh, Saudi Arabia;(10) Pontchaillou University, Rennes, France;(11) St. Thomas Hospital, London, UK;(12) Hospital 9 de Julho, Sao Paulo, Brazil;(13) South Hospital Amiens, Amiens, France;(14) Queen Elizabeth Hospital, London, UK;(15) Barwon Health, Geelong, VC, Australia;(16) Clinical Centre for Research Excellence in Infectious Diseases, Royal Melbourne Hospital, Melbourne, Australia;(17) Departments of Medicine and Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA;(18) ARUP Laboratories, Salt Lake City, UT, USA |
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| Abstract: | A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC
Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and
aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed
phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T
groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic
characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. |
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| Keywords: | Leptotrichia hongkongensis Bacterium Blood culture Anaerobic bacteria Novel species |
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