| Effect of cultivating conditions on α-galactosidase production by a novel Aspergillusfoetidus ZU-G1 strain in solid-state fermentation |
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| 作者姓名： | Liu CQ Chen QH Cheng QJ Wang JL He GQ |
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| 作者单位： | Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China |
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| 摘 要： | The work is intended to achieve optimum culture conditions of α-galactosidase production by a mutant strain ,Aspergillusfoetidus ZU-GI in solid-state fermentation （SSF）. Certain fermentation parameters involving moisture content, incubation temperature, cultivation period of seed, inoculum volume, initial pH value, layers of pledget, load size of medium and period of cultivation were investigated separately. The optimal cultivating conditions of α-galactosidase production in SSF were 60% initial moisture of medium, 28 ℃ incubation temperature, 18^h cultivation period of seed, 10% inoculum volume, 5.0-6.0 initial pH of medium, 6 layers of pledget and 10 g dry matter loadage. Under the optimized cultivation conditions, the maximum α-galactosidase production was 2037.51 U/g dry matter near the 144th hour of fermentation.
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| 关 键 词： | 臭曲霉 ZU-G1品系 培养条件 固态发酵 α-半乳糖苷酶生产 |
| 收稿时间： | 2006-05-13 |
| 修稿时间： | 2006-07-31 |
| 本文献已被 CNKI 维普 PubMed SpringerLink 等数据库收录！ |
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