Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection |
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| Authors: | Lang Qiu-lei Zhou Xiao-chuan Zhang Xiao-lin Drabek Rafal Zuo Zhi-xiang Ren Yong-liang Li Tong-bin Chen Ji-shuang Gao Xiao-lian |
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| Institution: | (1) College of Life Sciences, Zhejiang University, Hangzhou, 310058, China;(2) Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, 310018, China;(3) LC Sciences, 2575 W. Bellfort, Suite 270, Houston, TX 77054, USA;(4) Department of Biology and Biochemistry, University of Houston, Houston, TX 77004, USA; |
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| Abstract: | A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under
various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant
miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato
miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs
are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression
of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate
that developmental anomalies elicited by virus infection may be caused by more complex biological processes. |
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| Keywords: | Solanum lycopersicum Plant miRNA Cucumber mosaic virus miRNA array miRNA response |
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