Spinal cord decompression reduces rat neural cell apoptosis secondary to spinal cord injury |
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| Authors: | Kan Xu Qi-xin Chen Fang-cai Li Wei-shan Chen Min Lin Qiong-hua Wu |
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| Institution: | (1) Department of Orthopaedic Surgery, School of Medicine, Zhejiang University, Hangzhou, 310009, China;(2) Department of Pathology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China |
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| Abstract: | Objective: To determine whether spinal cord decompression plays a role in neural cell apoptosis after spinal cord injury. Study design: We used an animal model of compressive spinal cord injury with incomplete paraparesis to evaluate neural cell apoptosis after decompression. Apoptosis and cellular damage were assessed by staining with terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labelling (TUNEL) and immunostaining for caspase-3, Bcl-2 and Bax. Methods: Experiments were conducted in male Spragne-Dawley rats (n=78) weighing 300-400 g. The spinal cord was compressed posteriorly at T10 level using a custom-made screw for 6 h, 24 h or continuously, followed by decompression by removal of the screw. The rats were sacrificed on Day 1 or 3 or in Week 1 or 4 post-decompression. The spinal cord was removed en bloc and examined at lesion site, rostral site and caudal site (7.5 mm away from the lesion). Results: The numbers of TUNEL-positive cells were significantly lower at the site of decompression on Day l, and also at the rostral and caudal sites between Day 3 and Week 4 post-decompression, compared with the persistently compressed group. The numbers of cells between Day 1 and Week 4 were immunoreactive to caspase-3 and B-cell lymphoma-2 (Bcl-2)-associated X-protein (Bax), but not to Bcl-2, correlated with those of TUNEL-positive cells. Conclusion: Our results suggest that decompression reduces neural cell apoptosis following spinal cord injury. |
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| Keywords: | Spinal cord injury Decompression Apoptosis Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labelling (TUNEL) Caspase-3 B-cell lymphoma-2 (Bci-2) Bcl-2-associated X-protein (Bax) |
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