Isolation and characterization of a novel strain of Natrinema containing a bop gene |
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| Authors: | Xu Xue-wei Wu Min Huang Wei-da |
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| Institution: | School of Life Sciences, Zhejiang University, Hangzhou 310027, China. |
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| Abstract: | A novel member of extremely halophilic archaea, strain AJ2, was isolated from Ayakekum Lake located in Altun Mountain National Nature Reserve of Xinjiang Uygur Autonomous Region in China. The strain A J2 requires at least 10% (w/v)NaCl and grows 10% to 30% (optimum at 20%). Phylogenetic analysis based on 16S rDNA sequence comparison revealed that strain A J2 clustered to three Natrinema species with less than 97.7% sequence similarities, suggesting A J2 is a novel member of Natrinema. A bacteriorhodopsin-encoding (bop) gene was subsequently detected in the A J2 genome using the polymerase chain reaction technique. The cloning and sequencing of a 401 base pairs fragment indicated the deduced amino acid sequence of bop from A J2 is different from that reported for bacteriorhodopsins. This is the first reported detection of a bop gene in Natrinema. |
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| Keywords: | Halophilic archaea Phylogeny Bacteriorhodopsin |
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