Improved myocardial perfusion and cardiac function by controlled-release basic fibroblast growth factor using fibrin glue in a canine infarct model |
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| Authors: | Nie Shao-ping Wang Xiao Qiao Shi-bin Zeng Qiu-tang Jiang Ju-quan Liu Xiao-qing Zhu Xiang-ming Cao Guo-xiang Ma Chang-sheng |
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| Institution: | (1) Department of Cardiovascular Surgery, Hospital Universitario La Princesa, c/Diego de Leon 62, Madrid 28006, Spain;(2) Department of Cardiothoracic Surgery, Mid America Heart Institute, St Luke 's Hospital, Kansas City, Missouri, USA;(3) Department of Haematology, Hospital Universitario La Princesa, c/Diego de Leon 62, Madrid 28006, Spain;(4) Department of Radiology, Hospital Universitario La Princesa, c/Diego de Leon 62, Madrid 28006, Spain;(5) Department of Nuclear Medicine, Hospital Universitario La Princesa, c/Diego de Leon 62, Madrid 28006, Spain |
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| Abstract: | Objective: Angiogenic therapy is emerging as a potential strategy for the treatment of ischemic heart disease but is limited
by a relatively short half-life of growth factors. Fibrin glue (FG) provides a reservoir for controlled- release of growth
factors. The aim of this study was to evaluate the effects of basic fibroblast growth factor (bFGF) incorporating FG on angiogenesis
and cardiac performance in a canine infarct model. Methods: Acute myocardial infarction was induced by ligation of the left
anterior descending coronary artery (LAD). Group I (n=6) underwent ligation of LAD alone. In Group II, transmural channels were created in the infarct area (n=6). In Group III, non-transmural channels were created to locate FG cylinders containing bFGF (n=6). Eight weeks after operation, myocardial perfusion was assessed by single photon emission computed tomography, cardiac
function by echocardiography, and vascular development by immunohistochemical staining. Results: Total vascular density and
the number of large vessels (internal diameter ≥50 μm) were dramatically higher in Group III than in Groups I and II at eight
weeks. Only the controlled-release group exhibited an improvement in regional myocardial perfusion associated with lower defect
score. Animals in Group III presented improved cardiac regional systolic and diastolic functions as well as global systolic
function in comparison with the other two groups. Conclusions: Enhanced and sustained angiogenic response can be achieved
by controlled-release bFGF incorporating FG within transmyocardial laser channels, thus enabling improvement in myocardial
perfusion and cardiac function. |
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| Keywords: | Angiogenesis Basic fibroblast growth factor Controlled release Ischemic heart disease |
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