| A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor |
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| 作者姓名： | 李永泉 岑沛霖 陈时飞 吴丹 郑静 |
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| 作者单位： | College of Life Science，Institute of Bioengineering，Zhejiang University，Hongzhou 310027，China，College of Life Science，College of Life Science，College of Life Science，Drug Adiministration of Zhejiang Province，Hongzhou 310012，China |
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| 基金项目： | Project (No.20172046) supported by the National Natural Foun-dation of China |
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| 摘 要： | INTRODUCTION S.coelicolor as a model system has been ex-tensively studied with the aid of genetic and mo-lecular techniques. Its ability to produce four anti-biotics (Hopwood et al., 1995), namely actinorhodin (Act), undecylprodigiosin (Red), Calcium-Depend- ent Antibiotic (CDA) and methylenomycin (Mmy), enables studies of pathyway-specific and pleiotropic regulation of antibiotic production. In which, Red is a red-pigmented antibiotic and Act is coelicolor- pigmented antibiotic tha…
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A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor |
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| LI Yong-quan ,CEN Pei-lin,CHEN Shi-fei ,WU Dan ,ZHENG Jing.A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor[J].Journal of Zhejiang University Science,2004(2). |
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| Authors: | LI Yong-quan CEN Pei-lin CHEN Shi-fei WU Dan ZHENG Jing |
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| Institution: | LI Yong-quan 1,CEN Pei-lin2,CHEN Shi-fei 1,3,WU Dan 1,ZHENG Jing 1 |
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| Abstract: | Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Strep-tomyces coelicolor. DNA microarray analysis revealed that ecrA1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster. |
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| Keywords: | Streptomyces coelicolor Two-component system Antibiotic gene cluster ecrA1/A2 |
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