| 狗血浆中哌唑嗪的含量测定方法及其在药代动力学中的应用 |
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| 引用本文： | 张妮萍,余书勤,王辉,申艳艳,徐师,张玲,许茜.狗血浆中哌唑嗪的含量测定方法及其在药代动力学中的应用[J].东南大学学报,2011(2):217-221. |
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| 作者姓名： | 张妮萍 余书勤 王辉 申艳艳 徐师 张玲 许茜 |
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| 作者单位： | 东南大学环境医学工程教育部重点实验室;南京师范大学江苏省医药超分子及应用重点实验室;东南大学苏州研究院苏州市环境与生物安全重点实验室; |
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| 基金项目： | Pre-Research Foundation for the National Natural Science Foundation of Southeast University(No.9225000007); Suzhou Science and Technology Development Projects(No.YJS0948) |
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| 摘 要： | 建立并评价了一种简单灵敏的方法测定狗血浆中PZS的含量.该方法利用HPLC-FLD结合乙醚萃取的前处理方法,采用C18色谱柱(150 mm×4.6 mm,5 μm),流动相为30％乙腈和70％乙酸-乙酸钠缓冲液(pH=3.6),荧光检测器的激发波长为258 nm,发射波长为387 nm,在流动相流速为1.0 mL/mi...
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| 关 键 词： | 哌唑嗪 PZS-SBE-β-CD包合物片剂 高效液相色谱 药物代谢动力学 |
Method for quantification of prazosin in dog plasma and its application to pharmacokinetic study |
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| Zhang Niping Yu Shuqin Wang Hui Shen Yanyan, Xu Shi Zhang Ling Xu Qian.Method for quantification of prazosin in dog plasma and its application to pharmacokinetic study[J].Journal of Southeast University(English Edition),2011(2):217-221. |
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| Authors: | Zhang Niping Yu Shuqin Wang Hui Shen Yanyan Xu Shi Zhang Ling Xu Qian |
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| Institution: | Zhang Niping1 Yu Shuqin2 Wang Hui2 Shen Yanyan1,3 Xu Shi1 Zhang Ling1 Xu Qian1,3(1 Ministry of Education Key Laboratory of Environmental Medicine and Engineering,Southeast University,Nanjing 210009,China)(2 Jiangsu Key Laboratory for Medical Supermolecule Material and Application,Nanjing Normal University,Nanjing 210046,China)(3 Suzhou Key Laboratory of Environment and Biosafety,Suzhou Institute,Suzhou 215123,China) |
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| Abstract: | A simple and sensitive high performance liquid chromatography method using fluorescence detection(HPLCFLD) and a one-step single solvent extraction for the determination of prazosin(PZS) in dog plasma is developed and validated.After extraction with ether,the chromatographic separation of PZS is carried out using a reverse phase C18 column(150 mm×4.6 mm,5 μm) with a mobile phase of 30% acetonitrile and 70% acetic acid-sodium acetate buffer solution(pH = 3.6) and quantified by fluorescence detection operated with an excitation wavelength of 258 nm and an emission wavelength of 387 nm.The flow rate of the mobile phase is 1.0 mL /min and the retention time of PZS and the internal standard is found to be 4.4 and 5.8 min,respectively.The calibration curve is linear within a concentration range from 1.0 to 1 000.0 ng /mL(r2 > 0.998).The limit of detection is 0.4 ng /mL.The inter-day coefficient of variation(COV) of the calibration standards is below 5.0% and the mean accuracy is in the range from 92.7% to 104.2%.Moreover,by analyzing quality control plasma samples for three days,the results show that the method is precise and accurate,for the intra-and interday COV within 10% and the accuracy from 95.9% to 112.7%.The developed and validated method is successfully applied to pharmacokinetic study for the preclinical evaluation of a new peroral PZS-sulfobutyl ether beta-cyclodextrin(PZS-SBE-βCD) inclusion complex tablets(test preparation),which demonstrates that the test preparation released PZS is conducted in a slow and controlled way,and the relative bioavailability of the test preparation is found to be 105.0%. |
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| Keywords: | prazosin(PZS) PZS-sulfobutyl ether betacyclodextrin(PZS-SBE-β-CD) inclusion complex tablets high performance liquid chromatography(HPLC) pharmacokinetics |
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