| 耐受Pb^2＋细菌的筛选及对Pb^2＋吸附特性的研究 |
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| 引用本文： | 李辉,马丽,李华,屈波.耐受Pb^2＋细菌的筛选及对Pb^2＋吸附特性的研究[J].绵阳师范高等专科学校学报,2013(5):61-66,70. |
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| 作者姓名： | 李辉 马丽 李华 屈波 |
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| 作者单位： | 绵阳师范学院资源环境工程学院,四川绵阳621000 |
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| 摘 要： | 通过驯化培养从受Pb2＋污染的土壤中分离到1株能在含Pb2＋浓度为300 mg.L-1液体培养基中生长的耐Pb2＋细菌菌株（B19）,对该菌株吸附Pb2＋能力及影响吸附Pb2＋的因素进行了探讨.结果表明：该菌株对Pb2＋的吸附速率较快,在5 min内,培养液中Pb2＋去除率达到76.4%,在30 min达到吸附平衡.pH、培养基、菌量、Pb2＋初始质量浓度对去除率有显著影响.在吸附时间为30 min,吸附温度为30℃,菌龄为3 d,牛肉膏蛋白胨培养基培养,pH5.5,Pb2＋质量浓度85 mg.L-1,菌量6 g.L-1时,其对Pb2＋去除率可达92.6%.
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| 关 键 词： | 重金属 Pb2＋ 耐受性 生物吸附 筛选 |
On Screening Tolerating Bacteria Pb^2＋ and Its Adsorption |
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| LI Hui,MA Li,LI Hua,QU Bo.On Screening Tolerating Bacteria Pb^2＋ and Its Adsorption[J].Journal of Mianyang Teachers College,2013(5):61-66,70. |
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| Authors: | LI Hui MA Li LI Hua QU Bo |
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| Institution: | (School of Resource ＆ Environmental Engineering,Mianyang Normal University,Mianyang,Sichuan 62100) |
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| Abstract: | A bacterial strain,which could grow in a concentration of 300 mg·L-1Pb2＋,was separated from the Pb2＋contaminated soil by acclimatization and enrichment treatment.Pb2＋ biosorption acts and conditions were discussed.The results showed that the biosorption on Pb2＋ was a rapid process.The adsorptive rate reached 76.4% within the initial 5min and biosorption-equilibrilium period was 30 min.The effect of pH value,medium,biosorbent＇s dosag and the concentration of Pb2＋on adsorptive rate were remarkable.The removal rate reached 92.6% under the conditions of adsorptive time at 30 min,temperature at 30 ℃,biosorbent age at 3 d,in beef extract peptone medium,pH at 5.5,the Pb2＋ concentration at 85 mg·L-1,biosorbent dosage at 6 g·L-1. |
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| Keywords: | Heavy metal Pb2＋ tolerance biosorption screening |
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