| 坛紫菜单个体细胞培养的愈伤组织发育途径 |
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| 引用本文： | 胡银茂.坛紫菜单个体细胞培养的愈伤组织发育途径[J].台州学院学报,2006,28(3):67-69. |
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| 作者姓名： | 胡银茂 |
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| 作者单位： | 宁波教育学院科技分院,浙江,宁波,315010 |
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| 摘 要： | 用海螺酶酶解坛紫菜(Porphyra haitanensis)叶状体制备游离的营养细胞,在显微镜下选取单个细胞放入96孔板中进行隔离培养。一部分细胞发育成了愈伤组织状的细胞团。这些细胞团继续发育,最终形成了丝状体或小叶片。
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| 关 键 词： | 坛紫菜 单细胞 愈伤组织 |
| 文章编号： | 1672-3708（2006）03-0067-03 |
| 收稿时间： | 2006-03-23 |
| 修稿时间： | 2006年3月23日 |
The Morphogeny of Callus from Single Somatic Cell Clone Cultivation of Porphyra Haitanensis |
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| HU Yin-mao.The Morphogeny of Callus from Single Somatic Cell Clone Cultivation of Porphyra Haitanensis[J].Journal of Taizhou University,2006,28(3):67-69. |
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| Authors: | HU Yin-mao |
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| Institution: | Ningbo Institute of Education, Ningbo 315010, China |
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| Abstract: | The releases of free - living vegetative cells were achieved by enzymolysis of Porphyra haitanensis fronds, then single somatic cells were picked out into 96 - well microplates under invert microscope, with the help of capillary tube. The development of somatic cells was observed continuously and photos were taken. In this paper, the morphology of callus that developed from one cell was described. The final destiny of callus was conchocelia or small blades. |
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| Keywords: | Porphyra haitanensis single somatic cell callus |
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