| 槐角多糖及其水解物中单糖的检测 |
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| 引用本文： | 方艳夕,周丽丽,叶强,胡文斌.槐角多糖及其水解物中单糖的检测[J].安徽科技学院学报,2017,31(1). |
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| 作者姓名： | 方艳夕 周丽丽 叶强 胡文斌 |
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| 作者单位： | 安徽科技学院 食品药品学院,安徽 凤阳,233100;安徽科技学院 食品药品学院,安徽 凤阳,233100;安徽科技学院 食品药品学院,安徽 凤阳,233100;安徽科技学院 食品药品学院,安徽 凤阳,233100 |
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| 基金项目： | 安徽科技学院校级自然科学课题，安徽科技学院重点建设学科 |
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| 摘 要： | 目的:测定槐角中多糖的含量及其多糖水解物中单糖。方法:利用分光光度法测定槐角多糖含量。利用三氟乙酸制备多糖水解物,采用毛细管电泳-安培检测槐角多糖水解物中单糖。结果:槐角中多糖的含量为3.12%。槐角多糖水解物中含有果糖和葡萄糖,两者的摩尔比为34.2∶1。结论:该方法准确、简便,可用于槐角中多糖及其多糖水解物中单糖的检测。
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| 关 键 词： | 槐角多糖 毛细管电泳-安培检测 单糖 |
Content Determination and Monosaccharides in HydrolysateDetection of Sophorae Fructus Polysaccharides |
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| FANG Yan-xi,ZHOU Li-li,YE Qiang,HU Wen-bin.Content Determination and Monosaccharides in HydrolysateDetection of Sophorae Fructus Polysaccharides[J].Journal of Anhui Science and Technology University,2017,31(1). |
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| Authors: | FANG Yan-xi ZHOU Li-li YE Qiang HU Wen-bin |
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| Abstract: | Objective:The content of Sophorae Fructus polysaccharide(SFP) was determined.The monosaccharides were analysed in SFP hydrolysate.Method:The content of SFP was determined with spectrophotometry.SFP was hydrolyzed by trifluoroacetic acid(TFA)solution.Monosaccharide compositions in SFP were deter mined by Capillary electrophoresis-Amperometric detection(CE-AD) method.Results:The content of SFP was 3.12%.The hydrolysate of SFP was composed of fructose,glucose and so on.The molar ratio of fructose and glucose in SFP hydrolysate was about 34.2∶1.Conclusion:The method is accurate, simple and can be used for the determination of SFP and its hydrolysate. |
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| Keywords: | Sophorae Fructus polysaccharides Capillary electrophoresis-Amperometric detection Monosaccharides |
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