| 用百合叶片及其鳞茎叶片离体诱导不定芽 |
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| 引用本文： | 赵银萍,张九东,程建国.用百合叶片及其鳞茎叶片离体诱导不定芽[J].西安文理学院学报,2005,8(1):21-23. |
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| 作者姓名： | 赵银萍 张九东 程建国 |
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| 作者单位： | [1]西安文理学院生命科学系，陕西西安710065 [2]西北农林科技大学，陕西杨陵712100 |
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| 摘 要： | 以MS为基本培养基，附加不同浓度配比的NAA、6-BA、TDZ、2．4-D等激素，分别对5种百合的鳞片叶和叶片进行培养．结果显示，叶片经MS TDZ0．5mg／L和MS 2．4-D0．2mg／L TDZ2．0mg／L诱导可产生不定芽，诱导率最高为20％．MS NAA0．03mg／L、MS NAA0．05mg／L和MS 6-BA2．0mg／L 2．4-D0．2mg／L的培养基都不同程度诱导鳞片叶产生多个不定芽。以MS NAA0．05mg／L芽诱导率最高，不定芽经过继代培养，可长出新鳞茎．
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| 关 键 词： | 不定芽 叶片 离体诱导 鳞茎 百合 基本培养基 2．4-D 浓度配比 6-BA 继代培养 诱导率 NAA TDZ 鳞片 |
| 文章编号： | 1008-5564(2005)01-0021-03 |
| 修稿时间： | 2004年9月15日 |
In Vitro Induction of Adventitious Buds from Leaves and Scale Leaves of Lily Species |
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| ZHAO Yin-Ping,ZHANG Jiu-Dong,CHENG Jian-Guo.In Vitro Induction of Adventitious Buds from Leaves and Scale Leaves of Lily Species[J].Journal of Xi‘an University of Arts & Science:Natural Science Edition,2005,8(1):21-23. |
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| Authors: | ZHAO Yin-Ping ZHANG Jiu-Dong CHENG Jian-Guo |
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| Abstract: | The leaves and scale leaves of five lily species were cultivated on MS basic medium, supplemented with different concentration of NAA, 6-BA, TDZ and 2.4-D. The result showed that adventitious buds were induced directly from the leaves when they were cultivated on MS+TDZ 0.5 mg/L or MS+2.4-D 0.2 mg/L+TDZ 2.0 mg/L. The highest induction rate of adventitious bud was 20%. When the scale leaves were cultivated on MS+NAA 0.03 mg/L, MS+NAA 0.05 mg/L and MS+6-BA 2.0 mg/L+2.4-D 0.2 mg/L medium. Adventitious buds were inducted. The induction rate adventitious buds was the highest on MS+NAA 0.05 mg/L medium, theses adventitious buds could bring up new scale bulbs when they were continuously cultured. |
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| Keywords: | in vitro culture lily adventitiou bud |
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