| 酶解法辅助提取花椒油树脂的工艺优化 |
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| 引用本文： | 陶志杰,;王睿,;王改玲,;许晖,;朱晓霞,;孙晓侠.酶解法辅助提取花椒油树脂的工艺优化[J].绥化学院学报,2014(9):150-153. |
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| 作者姓名： | 陶志杰 ;王睿 ;王改玲 ;许晖 ;朱晓霞 ;孙晓侠 |
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| 作者单位： | [1]蚌埠学院,安徽蚌埠233030; [2]成都市青白江区农村发展局,四川成都610300 |
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| 基金项目： | 安徽高校省级自然科学研究项目（KJ20132198）,安徽省教育厅科研项目（KJ20098076）,安徽省高等学校省级质量工程项目（项目编号：20101094）. |
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| 摘 要： | 试验以纤维素酶法辅助优化提取花椒油树脂。通过利用单因素和正交试验分析,结果表明,酶解温度为影响提取效果的显著因子,优化后提取条件为酶用量2.0mg/100mL,酶解时间1.5h,酶解温度45℃,在该工艺条件下提取一次花椒油树脂的平均提取率为30.2%。验证试验表明,经酶解提取法提取花椒油树脂与未经酶解处理的乙醇浸提法相比,结果增长25.8%。
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| 关 键 词： | 花椒 油树脂 纤维素酶 正交试验 |
Optimization of Extraction Process Conditions of Oleoresin from Zanthoxylum bungeanum by Enzymatic Method |
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| Institution: | Tao Zhijie, Wang Rui, Wang Gailing, Xu Hui, Zhu Xiaoxia, Sun Xiaoxia ( 1.Department of Biology and Food Engineering, Bengbu CoUege,Bengbu Anhui, 233030, China 2.Qingbaijiang District Village Development Bureau,Chengdu Siehuan, 610300, China) |
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| Abstract: | The present study was conducted to optimize the extraction of oleoresin from Zanthoxylum bungeanum with cellulase. By using one-factor-at-a-time and orthogonal array design methods, the results showed that hydrolysis temperature was the significant factor, enzyme dose of 2.0 mg/100 mL, hydrolysis time of 1.5 h and hydrolysis temperature of 45℃. Under the optimum conditions, the extraction efficiency of oleoresin was 30.2%, 25.8%higher than the yield of ethanol extraction. |
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| Keywords: | Zanthoxylum bungeanum Oleoresin Cellulase Orthogonal experiment |
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