| 鞣花酸对前列腺癌PC-3细胞生长和凋亡的影响 |
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| 引用本文： | 郑英俊,阳宁,彭荣章,邓全红,邹保国.鞣花酸对前列腺癌PC-3细胞生长和凋亡的影响[J].孝感职业技术学院学报,2009,12(2):101-105. |
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| 作者姓名： | 郑英俊 阳宁 彭荣章 邓全红 邹保国 |
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| 作者单位： | 郑英俊,邓全红,邹保国,ZHENG Ying-jun,DENG Quan-hong,ZOU Bao-guo(荆门市第二人民医院,湖北,荆门,448001);阳宁,彭荣章,YANG Ning,PENG Rong-zhang(南华大学附二医院,湖南,衡阳,421001) |
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| 摘 要： | 目的观察鞣花酸对前列腺癌PC-3细胞株的影响。方法：体外培养人前列腺癌PC-3细胞，加入0μg/ml、2．5μg/ml、5μg/ml、10μg/ml、20μg/ml的鞣花酸作用于PC-3细胞，分别作用12小时、24小时和48小时后，应用MTr法测定各浓度组鞣花酸对PC-3细胞的生长抑制作用。应用流式细胞仪检测鞣花酸作用48小时后，PC-3细胞的周期时相变化及凋亡情况。应用免疫细胞化学检测10μg/ml鞣花酸作用24小时后，实验组与对照组细胞Caspase-3蛋白表达情况。结果：鞣花酸明显抑制PC-3细胞的生长，抑制效应呈时间依赖型和浓度依赖型，与对照组比较均有统计学意义（P〈0．05）。应用流式细胞仪检测结果显示，鞣花酸可将PC-3细胞阻滞于G1／S期，并诱导PC-3细胞凋亡。免疫细胞化学结果显示实验组Ctmpase-3蛋白表达明显升高。结论：鞣花酸对前列腺癌PC-3细胞株有明显的生长抑制和诱导凋亡作用。
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| 关 键 词： | 鞣花酸 前列腺肿瘤 PC-3细胞 |
The Effect of Ellagic Acid on Prostate Cancer Cell Line PC -3 |
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| ZHENG Ying-jun,YANG Ning,PENG Rong-zhang,DENG Quan-hong,ZOU Bao-guo.The Effect of Ellagic Acid on Prostate Cancer Cell Line PC -3[J].Journal of Xiaogan Vocational-Technical College,2009,12(2):101-105. |
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| Authors: | ZHENG Ying-jun YANG Ning PENG Rong-zhang DENG Quan-hong ZOU Bao-guo |
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| Institution: | ZHENG Ying - jun, YANG Ning, PENG Rong - zhang, DENG Quan - hong, ZOU Bao - guo (1. The Second People＇s Hospital of Jingmen Jingmen ,Hubei 448000 ; 2. The Second Affiliated Hospital of Nanhua University ,Hengyang ,Hunan 421001 ) |
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| Abstract: | Objective： To observe the effect of Ellagic Acid on prostate cancer cell llne PC - 3. Methods： The human prostate cancer PC -3 cells were treated in vitro with EA in 0μg/ml,2.5μg/ml,5μg/ml, 10μg/ml, 20μg/ml for 12,24 ,48 hours. The cell viability was detected by MTr in all groups. The cell cycle arrest and apoptosis was detected by flow cytometry when PC - 3 cells were treated with EA of diferent conccrtration in the 48 hours. The immunocytochemistry method was used to assess the expression of Caspase - 3 protein when PC - 3 cells were treated with EA 10μg/ml for 24 hours. Results：EA significantly inhibited the pmroliferation and viability of the PC -3 cells in a time and dose dependent manner compared with the untreated control groups （all P 〈 0.05）. Flow cytometric analysis revealed that treatment with EA resulted in cell - cycle arrest in the G1 phase and increasing the percentages of apoptetic ceils in a dose - dependent manner. The immtmocytochemistry showed that Caspase -3 expression was higher compared with control groups, when PC -3 cells were treated with EA. Conclusions：E1- lagic Acid could enhance the growth suppression and induce apoptosis of PC -3 ceils. |
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| Keywords: | Ellagic Acid Prostatic Neoplasms PC- 3 Cell |
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