| 黄斑姜中期染色体CPD染色和45S rDNA荧光原位杂交分析 |
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| 引用本文： | 赵丽娟,郭敏.黄斑姜中期染色体CPD染色和45S rDNA荧光原位杂交分析[J].怀化学院学报,2011,30(2):45-47. |
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| 作者姓名： | 赵丽娟 郭敏 |
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| 作者单位： | 1. 怀化学院,生命科学系,湖南怀化418008;怀化学院,民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008;怀化学院,湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008
2. 怀化学院,生命科学系,湖南怀化418008 |
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| 摘 要： | 为了对黄斑姜(Zingiber flavo-maculatum S.Q.Tong.)的染色体进行识别并对该物种基因组的结构进行初步研究,利用PI和DAPI组合(CPD)染色和45S rDNA探针荧光原位杂交对中期染色体进行了分析.结果显示,黄斑姜具有2对45S rDNA位点,分别位于第3、4号染色体的短臂,对应于相应染...
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| 关 键 词： | 黄斑姜 核型 CPD染色 45S rDNA 荧光原位杂交 |
Analysis of Mitotic Metaphase Chromosomes of Zingiber flavo-maculatum using CPD Staining and FISH with 45S rDNA Probe |
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| ZHAO Li-juan,GUO Min.Analysis of Mitotic Metaphase Chromosomes of Zingiber flavo-maculatum using CPD Staining and FISH with 45S rDNA Probe[J].Journal of Huaihua University,2011,30(2):45-47. |
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| Authors: | ZHAO Li-juan GUO Min |
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| Abstract: | In order to identify the chromosomes of Zingiber flavo-maculatum S.Q.Tong.and reveal preliminarily the genome organization,CPD(combined PI and DAPI)staining and FISH with 45S rDNA probe were applied to analyze the mitotic metaphase chromosomes.Two pairs of 45S rDNA sites were detected on the short arms of the chromosome 3 and 4,respectively,corresponding to the prominent red CPD bands.The molecular cytogenetic karyotypes of Zingiber flavo-maculatum was constructed based on the data of rDNA and chromosome measurements.The karyotype formula is 2n=2X=22=12m+6sm+4st(SAT)and the karyotype asymmetry belongs to 2B type. |
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| Keywords: | 45S rDNA |
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