| 球毛壳菌素F对Poly（I：C）诱导的RAW264．7细胞TNF-α水平的影响 |
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| 引用本文： | 赵俊丽.球毛壳菌素F对Poly（I：C）诱导的RAW264．7细胞TNF-α水平的影响[J].南京晓庄学院学报,2011(6). |
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| 作者姓名： | 赵俊丽 |
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| 作者单位： | 南京晓庄学院生物化工与环境工程学院,江苏南京,211171 |
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| 摘 要： | 该实验主要研究球毛壳菌素F对聚肌苷酸胞苷酸Poly（I：C）诱导的小鼠巨噬细胞系RAW264．7 TNF-α表达水平的影响．采用不同浓度的Poly（I：C）（50μg·mL-1、100μg·mL-1和200μg·mL-1）和球毛壳菌素F（2μg·mL-1）对RAW264．7进行处理，运用流式细胞术检测细胞内TNF-α的表达水平．结果表明，各浓度的Poly（I：C）与对照组相比均能极显著提高RAW264．7的TNF-α水平（P〈0．001），并且具有浓度依赖性；球毛壳菌素F能够极显著地降低Poly（I：C）诱导的细胞TNF．O／水平（P〈0．01）．结论，球毛壳菌素F可能是通过降低炎症因子TNF-α的分泌而达到抗炎作用的．
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| 关 键 词： | 球毛壳菌素F 聚肌苷酸胞苷酸 肿瘤坏死因子α 小鼠巨噬细胞系RAW264．7 |
The Effect of Chaetoglobosin F on TNF-α Level by RAW264.7 Cells Induced by Poly（ I： C） |
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| Abstract: | The present study is to investigate the influence of chaetoglobosin F on TNF-α level induced by Poly（ I： C）. RAW264.7 cells were treated with various concentrations of Poly （ I： C ） （50μg/ml, 100μg/ml and 200μg/ ml） and chaetoglobosin F and then TNF-α expression levels of RAW264.7 were analyzed by flow eytometry. The result showed that all doses of Poly（ I： C） enhanced TNF-α production significantly compared with the control group with a dose dependent manner（ P 〈 0. 001 ）. Chaetoglobosin F decreased significantly the TNF-α expression of RAW264.7 cells induced by Poly（ I： C） （P 〈0.01 ）. It is concluded that ehaetoglobosin F plays an anti-inflammatory role partly through decreasing the secretion of TNF-α . |
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| Keywords: | chaetoglobosin F Poly（ I： C） TNF-α murine macrophage cell line RAW264 7 |
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