| 虎纹凤梨的组织培养与快速繁殖研究 |
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| 引用本文： | 张伟,何永.虎纹凤梨的组织培养与快速繁殖研究[J].南阳师范学院学报,2006,5(3):63-64. |
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| 作者姓名： | 张伟 何永 |
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| 作者单位： | 信阳师范学院,生命科学学院,河南,信阳,464000 |
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| 摘 要： | 用虎纹凤梨的侧芽作外植体进行培养,成功地建立了虎纹凤梨的快速繁殖程序。虎纹凤梨的侧芽基部切块接种在(1)Ms BA 1.5 mg/L诱导培养基上,形成愈伤组织;愈伤组织在(2)Ms BA 1.0 mg/L IBA 0.5 mg/L的培养基上诱导丛生芽及进行继代增殖培养,效果较好;不定芽在(3)Ms IBA 0.5 mg/L NAA 0.5 mg/L的培养基上易生根,健壮的试管苗移栽2个月后,成活率达98%。
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| 关 键 词： | 虎纹凤梨 组织培养 快速繁殖 |
| 文章编号： | 1671-6132（2006）03-0063-02 |
| 收稿时间： | 2005-05-18 |
| 修稿时间： | 2005年5月18日 |
Tissue culture and rapid propagation of Vriesea splanens |
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| ZHANG Wei,HE Yong.Tissue culture and rapid propagation of Vriesea splanens[J].Journal of Nanyang Teachers College,2006,5(3):63-64. |
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| Authors: | ZHANG Wei HE Yong |
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| Abstract: | A method for high frequency propagation of Vriesea splanens was established using the Vriesea splanens lateral bud as the explant.The results showed that the appropriate media for different culture stages were:(1)Ms BA (1.5 mg/L) for germination of the lateral bud.(2)Ms BA 1.0 mg/L IBA 0.5 mg/L for rapid propagation.(3)Ms IBA 0.5 mg/L NAA 0.5 mg/L for rooting.Survival rates reached 98% in two months for the planting the strong plant of test tube. |
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| Keywords: | Vriesea splanens tissue culture rapid propagation |
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