| 苹果皮孔斑点病的病原菌分离与鉴定 |
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| 引用本文： | 黄思良,于晶晶,荆新蕊,卢维宏,杨辉辉,陶爱丽.苹果皮孔斑点病的病原菌分离与鉴定[J].南阳师范学院学报,2013(12):33-36. |
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| 作者姓名： | 黄思良 于晶晶 荆新蕊 卢维宏 杨辉辉 陶爱丽 |
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| 作者单位： | [1]南阳师范学院生命科学与技术学院,河南南阳473061 [2]广西大学农学院,广西南宁530005 |
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| 基金项目： | 河南省科技创新团队建设项目（No.2010JRTSTHN012） |
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| 摘 要： | 2010年2月下旬,从南阳市超市购买的苹果中,发现感染皮孔斑点病的病果,从病果上随机采集病斑,经表面消毒后将病斑置于PDA平板上进行病原菌分离.从19个病斑中共分离获得17株形态相同的链格孢属真菌,经单孢纯化后对代表性致病菌株AP-5-3进行病原学鉴定.根据病原菌的形态特征及其核糖体基因内转录间隔区(rDNA-ITS)序列分析,将菌株AP-5-3鉴定为细交链孢菌(Alternaria alternata).该菌的最适生长温度为28℃,最适合生长pH为6.5.本文对苹果皮孔斑点病的病因进行了讨论.
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| 关 键 词： | 苹果 皮孔斑点病 病原鉴定 生物学特性 |
Isolation and identification of the causal agent of apple lenticel spot disease |
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| HUANG Si-liang YU Jing-jing JING Xin-rui LU Wei-hong YANG Hui-hui,TAO Ai-li.Isolation and identification of the causal agent of apple lenticel spot disease[J].Journal of Nanyang Teachers College,2013(12):33-36. |
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| Authors: | HUANG Si-liang YU Jing-jing JING Xin-rui LU Wei-hong YANG Hui-hui TAO Ai-li |
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| Institution: | 1. School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China; 2. College of Agriculture, Guangxi University, Nanning 530005, China) |
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| Abstract: | A few diseased fruit with lenticel spot symptoms were found among the apples purchased from a supermarket in Nanyang city in late February 2010. Lesions were randomly sampled from an affected apple, and used for pathogen isolation after surface disinfection. A total of 17 Alternaria isolates showing the same morphology were isolated exclusively from 19 apple lenticel lesions. One representative isolate AP- 5 -3 was used for etio- logical characterization after being purified by single spore isolation. The isolate was identified as Alternaria alternata based on its morphological characteristics and rDNA - ITS （internal transcribed spacer） sequence analysis. The most suitable temperature and pH for the growth of the pathogen were 28 ℃ and 6.5, respectively. The causal agent of apple lenticel spot disease was discussed in the paper. |
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| Keywords: | apple lenticel spot disease pathogen identification biological character |
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