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| SEREX技术筛选EID3及其表达的实验研究 | |
| 引用本文： | 王钦富,佟凤芝,刘希龙,唐玲,蒋革,河上裕.SEREX技术筛选EID3及其表达的实验研究[J].大连大学学报,2006,27(6):62-66. |
| 作者姓名： | 王钦富 佟凤芝 刘希龙 唐玲 蒋革 河上裕 |
| 作者单位： | 1. 大连大学,生物工程学院,辽宁,大连,116622 2. 大连大学,附属新华医院,辽宁,大连,116021 3. 日本庆应大学先端医学研究所 |
| 摘 要： | 目的]新的大肠癌相关性抗原EID3的基因克隆及其诊断价值研究.方法]利用大肠癌病人体内血清中所含的对肿瘤抗原产生的特异性抗体筛选睾丸组织cDNA噬菌体表达文库和大肠癌组织cDNA噬菌体表达文库(SEREX),并用RT-PCR技术研究EID3 mRNA在正常组织和大肠癌传代细胞表达.结果]睾丸组织cDNA噬菌体表达文库筛选得到了可以诱导大肠癌病人抗体免疫应答的新抗原EID3基因(Gen-bank NM_001008394.1).它们定位于染色体19q13.2,EID3含1个外显子.通过RT-PCR分析发现,EID3基因在43例大肠癌传代细胞株中,39例阳性,阳性率为90.7%.在正常组织中,除睾丸组织外不表达或有极低水平转录.结论]EID3 mRNA表达检测用于诊断大肠癌,可能具有高特异性和高敏感性的特点.EID3蛋白被首次发现在大肠癌病人中能够诱导机体的抗体免疫应答,为一个新的大肠癌相关性抗原分子.其功能可能与抑制细胞的恶性增殖相关,并可进一步研究其用于治疗和诊断大肠癌的可行性. |
| 关 键 词： | 大肠癌 EID3 SEREX 基因克隆与表达 |
| 文章编号： | 1008-2395（2006）06-0062-05 |
| 收稿时间： | 2006-10-21 |
| 修稿时间： | 2006年10月21 |
Cloning and expression of the gene encoding a novel colon cancer associated antigen-EID3 |
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| WANG Qin-fu,TONG Feng-zhi,LIU Xilong,TANG Ling,JIANG Ge,YUTAKA Kawakami.Cloning and expression of the gene encoding a novel colon cancer associated antigen-EID3[J].Journal of Dalian University,2006,27(6):62-66. | |
| Authors: | WANG Qin-fu TONG Feng-zhi LIU Xilong TANG Ling JIANG Ge YUTAKA Kawakami |
| Abstract: | Object] To clone the gene encoding a novel colon cancer associated antigen-EID3,and to investigate its mRNA expression pattern in normal tissue and colon cancer cell lines.\Screening on testis cDNA library and colon cancer cDNA library with the serum of patient with colon cancer,and investigating EID3 mRNA expression pattern in normal tissue and colon cancer cell lines by RT-PCR technique.\The gene encoding EID3 is located on chromosome 19q13.2,with one exon,the results of RT-PCR indicated that 39 out of 43 colon cancer cell lines were positive(90.7%),and the EID3 mRNA expression in normal tissue was exclusively positive in testis,other normal tissues were negative or very weak.\EID3 mRNA detection can be used in diagnosis of colon cancer with the possibility of high sensitivity and high specificity.It was the first time to discover that the EID3 protein can initiate humoral immune response in colon cancer patient.The function of EID3 may be associated with the inhibition of malignant cellular proliferation.It is necessary to further investigate the possibility of using EID3 in diagnosis and therapy of the patient with colon cancer. |
| Keywords: | colon cancer EID3 SEREX gene cloning and expression |
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