| 北五味子果实中多糖的提取与纯化研究 |
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| 引用本文： | 张兰杰,张维华,赵珊红.北五味子果实中多糖的提取与纯化研究[J].鞍山师范学院学报,2002,4(1):58-60. |
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| 作者姓名： | 张兰杰 张维华 赵珊红 |
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| 作者单位： | 鞍山师范学院,化学系,辽宁,鞍山,114005 |
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| 摘 要： | 经提取、醇析、Savage法脱蛋白、脱脂、脱色、DEAE纤维素柱色谱等步骤,从北五味子中分离出两种多糖组分,并经紫外光谱、红外光谱确证纯度.北五味子中粗多糖含量6.1%,三次提取率分别为:55.6%,38.5%,4.6%,Savage法脱蛋白粗多糖损失率为7.2%,北五味子中两种纯多糖含量分别为0.387%,0.061%.
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| 关 键 词： | 北五味子 多糖 提取 红外光谱 紫外光谱 纯化 |
| 文章编号： | 1008-2441(2002)01-0063-03 |
| 修稿时间： | 2001年10月5日 |
Study on the Extraction and Purification of Polysaccharide from Schisandra Chinensis(Turcz) Baill Fruit |
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| ZHANG Lan-jie,ZHANG Wei-hua,ZHAO Shan-hong.Study on the Extraction and Purification of Polysaccharide from Schisandra Chinensis(Turcz) Baill Fruit[J].Journal of Anshan Teachers College,2002,4(1):58-60. |
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| Authors: | ZHANG Lan-jie ZHANG Wei-hua ZHAO Shan-hong |
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| Abstract: | In this paper,two polysaccharides verified by UV-VIS and infrared spectroscopy,were obtained through the following steps:ext raction,ethanol precipitation,deproteinization,decolorization and column chro matography by DEAE cellulose. Quantification result of polysaccharide in Schisan dra Chinensis(Turcz)Baill Fruit show that crude polysaccharide content percent age in it is 6.1%, the three extraction ratio are 55.6%,38.5% and 4.8% respectiv ely ,the loss of crude polysaccharide during deproteinization by Savage method i s 7.2% and the percentages of the pure polysaccharides in Schisandra Chinensis (Turcz ) Baill Fruit are 0.387% and 0.06% . |
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| Keywords: | Schisandra Chinensis(Turcz) Baill Polysaccharide Extract ion IR UV-VIS Purification |
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