| 加拿大杨茎段愈伤组织诱导及不定芽分化研究 |
| |
| 引用本文： | 花阳景,林碧瑜,张智勇,游佳琪,刘希华.加拿大杨茎段愈伤组织诱导及不定芽分化研究[J].三明高等专科学校学报,2014(2):79-83. |
| |
| 作者姓名： | 花阳景 林碧瑜 张智勇 游佳琪 刘希华 |
| |
| 作者单位： | 三明学院资源与化工学院,福建三明365004 |
| |
| 基金项目： | 福建省大学生创新项目（201311311024）;福建省科技厅重点项目（2011N0030）;福建省教育厅科技项目（JA11249）. |
| |
| 摘 要： | 以加拿大杨茎段为外植体探讨不同激素处理对愈伤组织诱导及不定芽形成的影响.研究表明,在外植体消毒中,以0.1％升汞处理4.5min时间效果最好,以MS培养基＋0.15mg·L-1TDZ＋ 0.1mg·L-1NAA＋1.0mg·L-16-BA的愈伤组织诱导率最高达60％.丛生芽诱导最佳培养基为MS培养基＋0.15mg·L-1TDZ＋0.125mg·L-1NAA＋1.25mg·L-16-BA,丛生芽诱导率达86.7％.
|
| 关 键 词： | 加拿大杨 愈伤组织 不定芽 组织培养 |
Callus Induction and Adventitious Bud Regeneration from the Stem of Populus Canadensis |
| |
| HUA Yang-jing,LIN Bi-yu,ZHAN Zhi-yong,YOU Jia-ji,LIU Xi-hua.Callus Induction and Adventitious Bud Regeneration from the Stem of Populus Canadensis[J].Journal of Sanming College,2014(2):79-83. |
| |
| Authors: | HUA Yang-jing LIN Bi-yu ZHAN Zhi-yong YOU Jia-ji LIU Xi-hua |
| |
| Institution: | (College of Resources and Chemical Engineering, Sanming University, Sanming 365004, China) |
| |
| Abstract: | Taking the stem ofPopulus canadensis as a material, the effect for callus induction and bud differentiation with different phytohormone was studied. The result showed that the time effect for the explants dealing with 0.1% Hgcl2 about 4.5 min was the best. The most suitable formula for induction of callus was MS＋0.15mg .LITDZ＋ 0.1mg-L＇INAA＋ 1.0mg-L＇L6-BA, callus induction rate was 60%. The most suitable formula for shoot-inducing medium was MS ＋0.15mg. L^-1TDZ＋0.125rag. L^-1NAA＋l.25mg. Lt 6-BA, Multiple shoot clumps induction rate was 86.7%. |
| |
| Keywords: | Populus canadens/s callus adventitious bud tissue culture |
| 本文献已被 维普 等数据库收录！ |
|
