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| 中成药可溶性汞形态分析方法及样品结果测定 | |
| 引用本文： | 徐陆正,解清,闫赖赖,陈玉红,王京宇.中成药可溶性汞形态分析方法及样品结果测定[J].实验技术与管理,2011,28(12):40-43. |
| 作者姓名： | 徐陆正 解清 闫赖赖 陈玉红 王京宇 |
| 作者单位： | 1. 北京大学公共卫生学院,北京,100191 2. 北京大学公共卫生学院,北京100191;北京大学医药卫生分析中心,北京 100191 3. 安捷伦科技(中国)有限公司,北京,100102 |
| 基金项目： | "重大新药创制"科技重大专项课题 |
| 摘 要： | 建立了微波辅助提取、HPLC-ICP-MS联用分析中成药中二价汞和甲基汞的方法。分别比较了提取液中盐酸和L-半胱氨酸含量、提取温度和提取时间对提取结果的影响,得到了微波辅助提取的最优条件:体积分数为1%的盐酸10mL和质量分数为5%的L-半胱氨酸1mL作为提取液,控温70℃,微波辅助提取1h。该方法测得的二价汞和甲基汞的精密度分别为1.7%和4.4%,加标回收率分别为80.7%～99.5%和93.7%～95.8%。并应用该方法分析测定了20种、共49批次的含朱砂的中成药中可溶性汞的形态。 |
| 关 键 词： | 中成药 可溶性汞 形态分析 |
Speciation methods and sample analysis of soluble mercury in traditional Chinese medicines |
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| Xu Luzheng,Xie Qing,Yan Lailai,Chen Yuhong,Wang Jingyu.Speciation methods and sample analysis of soluble mercury in traditional Chinese medicines[J].Experimental Technology and Management,2011,28(12):40-43. | |
| Authors: | Xu Luzheng Xie Qing Yan Lailai Chen Yuhong Wang Jingyu |
| Institution: | Xu Luzheng1,Xie Qing1,2,Yan Lailai1,Chen Yuhong3,Wang Jingyu1,2(1.School of Public Health,Peking University,Beijing 100191,China,2 Center of Medical & Health Analysis,3 Agilent Technologies(China) Co.,Ltd.,Beijing 100102,China) |
| Abstract: | A method with microwave-assisted extraction and HPLC-ICP-MS to analyse the inorganic mercury and methyl mercury in the traditional Chinese medicines(TCMs) has been built.The hydrochloric acid and L-cysteine concentration,the extraction temperature and extraction time have been investigated.The optimal conditions are as follows: 1% hydrochloric acid 10 ml and 5% L-cysteine 1 ml as extract,temperature control 70 ℃,and time control 1h.Using this method,the inorganic mercury and methyl mercury’s RSDs(relative standard deviation) are 1.7% and 4.4%,and the standardized recoveries are 80.7~99.5% and 93.7~95.8%.Then,49 batches of 20 kinds TCMs which contain cinnabar have been determinated using this method. |
| Keywords: | traditional Chinese medicines soluble mercury speciation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |