| 辛基酚暴露对凡纳滨对虾NAGase的活力影响 |
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| 引用本文： | 谢晓兰,吴优,高平章,彭世旺,蒋乐杰.辛基酚暴露对凡纳滨对虾NAGase的活力影响[J].泉州师范学院学报,2014(6):30-33. |
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| 作者姓名： | 谢晓兰 吴优 高平章 彭世旺 蒋乐杰 |
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| 作者单位： | 泉州师范学院 化学与生命科学学院,福建 泉州,362000 |
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| 基金项目： | 福建省自然科学基金资助项目，国家自然科学基金资助项目 |
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| 摘 要： | 以凡纳滨对虾为研究对象,让其暴露在含0,5,15,45,135μg/L辛基酚（OP）的养殖水体中,暴露24,48,72,96,120 h后取样,分析测定凡纳滨对虾壳膜与内脏 NAGase 比活力的变化．结果表明,水体 OP 对对虾壳膜 NAGase比活力基本起激活作用．暴露72 h内,壳膜 NAGase比活力无显著性变化；暴露96 h时,5,15,135μg/L OP均对虾壳膜 NAGase 比活力起极显著激活作用（P＜0．01）,45μg/L OP 对虾壳膜 NAGase比活力起显著激活（P＜0．05）；暴露120 h时,15,135μg/L OP 对虾壳膜 NAGase 比活力仍表现为显著性激活作用（P＜0．05）,但5,45μg/L OP 对壳膜 NAGase 的比活力影响无显著性．而水体 OP 对虾内脏 NAGase比活力基本起抑制作用．在15μg/L OP体系下暴露24 h,内脏NAGase极显著下降（P＜0．01）,其他 OP浓度下无显著性变化；不同浓度下暴露48 h,对虾内脏 NAGase比活力均出现极显著下降（P＜0．01）；5μg/L OP体系下暴露72 h,对虾内脏 NAGase比活力无显著性下降,其他 OP 浓度下的对虾内脏 NAGase 比活力均出现极显著下降（P＜0．01）；15,135μg/L OP 体系下暴露96 h,内脏 NAGase 比活力也分别出现极显著（P＜0．01）及显著性（P＜0．05）下降．可见,OP暴露对对虾生长发育会产生影响．
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| 关 键 词： | 凡纳滨对虾 辛基酚 酶活力 |
Effects of Octyl-phenol on the Activity of N-Acetylglucosaminidase from Prawn(Litopenaeus vannamei) |
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| XIE Xiao-lan,WU You,GAO Ping-zhang,PENG Shi-wang,JIANG Le-jie.Effects of Octyl-phenol on the Activity of N-Acetylglucosaminidase from Prawn(Litopenaeus vannamei)[J].Journal of Quanzhou Normal College,2014(6):30-33. |
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| Authors: | XIE Xiao-lan WU You GAO Ping-zhang PENG Shi-wang JIANG Le-jie |
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| Institution: | (School of Chemistry and Life Sciences, Quanzhou Normal University, Fujian 362000,China) |
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| Abstract: | The effects of octyl-phenol （OP）on N-acetylglucosiminidase （NAGase）activity in the epidermis and hepatopancreas were investigated after prawns （Litopenaeus vannamei）were exposured to the different octyl-phenol concentrations（0,5,15,45,135μg/L）for different times（24,48,72,96, 120 h）,respectively.The results showed that OP could mainly activate NAGase in the epidermis.OP at the different concentrations had not significantly effect on the activity of the epidermal NAGase of prawns exposed for 72 hours,OP at 5,15 and 135μg/L extremely significantly activated and OP at 45μg/L significantly activated the epidermal NAGase of prawns exposed for 9 6 hours,respectively.After a 120 hours exposure to 15 and 135μg/L OP,there were still significant increases in the activity of the epidermal NAGase.However,OP could mainly inhibit the hepatopancreatic NAGase.After a 24 hours exposure to 1 5μg/L OP,there was extremely significant decrease in the activity of hepatopancreatic NAGase.OP at 5,15,45 and 135μg/L extremely significantly inhibited the hepatopancreatic NAGase of prawns exposed for 48 hours.OP at 15,45 and 135μg/L extremely significantly inhibited the hepa-topancreatic NAGase of prawns exposed for 72 hours.OP at 15 and 135μg/L extremely significantly and significantly inhibited the hepatopancreatic NAGase of prawns exposed for 9 6 hours,respectively. |
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| Keywords: | Litopenaeus vannamei N-acetylglucosiminidase Octyl-phenol Enzyme activity |
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