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南方碱蓬叶总黄酮微波-碱水法提取工艺
引用本文:林文谋,黄晓冬,黄晓昆,李裕红,缪翠艳.南方碱蓬叶总黄酮微波-碱水法提取工艺[J].泉州师范学院学报,2013,31(2):79-83.
作者姓名:林文谋  黄晓冬  黄晓昆  李裕红  缪翠艳
作者单位:泉州师范学院湿地研究所,福建泉州,362000
摘    要:采取微波-碱水法提取泉州湾产南方碱蓬叶总黄酮(LFSA),以紫外分光光度法所测定的总黄酮提取率为指标,采用单因素实验与5因素4水平L16(45)正交实验筛选碱水的pH值、微波功率、液料比、微波处理时间4个因素的4个水平的最佳组合.结果表明:碱水的pH值对LFSA提取率有显著影响(P〈0.05),LFSA微波-碱水法提取的最佳工艺条件为pH13、微波功率600W、液料比70、微波处理时间9min、提取次数1次,在此条件下,总黄酮提取率为4.87%.

关 键 词:泉州湾湿地  南方碱蓬叶  总黄酮  微波-碱水法

Study on Microwave-alkali Water Extracting Technics of Total Flavone from Suaeda Australis Leaves
LIN Wen-mou , HUANG Xiao-dong , HUANG Xiao-kun , LI Yu-hong , MIU cui-yan.Study on Microwave-alkali Water Extracting Technics of Total Flavone from Suaeda Australis Leaves[J].Journal of Quanzhou Normal College,2013,31(2):79-83.
Authors:LIN Wen-mou  HUANG Xiao-dong  HUANG Xiao-kun  LI Yu-hong  MIU cui-yan
Institution:(Institute of Wetland, Quanzhou Normal University, Fujian 362000, China)
Abstract:Total flavones of Suaeda australis leaves (LFSA) in Quanzhou Bay Wetland were extracted by the microwave-alkali water method. Taking the extraction rate of total flavone determined by ultraviolet spectrophotometry as an inspecting index, the microwave-alkali water optimization of water pH, microwave power,ratio of liquid to material and time of microwave treatment microwave- alkali water extraction process were achieved by single experiment and L16 (45 ) orthogonal test. The results showed that alkali water pH has significant effect on the extraction rate of total flavonoids.the optimum extraction conditions of microwave-alkali water method are as follows, the alkali water pH value being 13 ,the microwave power as 600 wattage,ratio of liquid to material being 70, microwave treatment time as 9 minute and making the extraction for one time. Under such condition, the extraction rate of total flavones is 4.87 %.
Keywords:Quanzhou bay wetland  Suaeda australis leaves flavone microwave-alkali water extractionmethod
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