| 一株拮抗细菌拮抗性能的测试与评价 |
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| 引用本文： | 贾翠英,王秀梅,张玉辉.一株拮抗细菌拮抗性能的测试与评价[J].河南职业技术师范学院学报,2011(2):38-42. |
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| 作者姓名： | 贾翠英 王秀梅 张玉辉 |
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| 作者单位： | [1]河南科技学院,河南新乡453003 [2]三门峡市湖滨果汁有限公司,河南三门峡472000 |
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| 基金项目： | 河南科技学院高等人才创新项目（2009Y-027002） |
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| 摘 要： | 以测定的抑菌圈直径大小判定拮抗细菌的拮抗性能,并通过单因素及正交试验对拮抗细菌发酵培养基组分及发酵条件进行设计与优化.结果表明,最优化发酵培养基组成为淀粉3 g/L,蛋白胨12 g/L,氯化钙3 g/L,利用最佳发酵培养基进行条件为初始pH 8.0,接种量8%,装液量75 mL/250 mL,发酵时间24 h的发酵,此拮抗细菌的拮抗性能最佳,抑菌圈直径达2.6 cm左右.该研究为进一步提高拮抗细菌拮抗性能提供一定参考数据,同时也为拮抗细菌更好地应用提供必要的理论依据.
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| 关 键 词： | 拮抗细菌 抑菌圈 培养基优化 |
Testing and evaluation of antagonistic performance for an antagonistic bacteria strain |
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| Jia Cuiying,Wang Xiumei,Zhang Yuhui.Testing and evaluation of antagonistic performance for an antagonistic bacteria strain[J].Journal of Henan Vocation-Technical Teachers College,2011(2):38-42. |
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| Authors: | Jia Cuiying Wang Xiumei Zhang Yuhui |
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| Institution: | 1.Henan Institute of Science and Technology,Xinxiang 453003,China;2.Hubin Beverage Limited Company of Sanmenxia,Sanmenxia 472000,China) |
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| Abstract: | Measuring the diameters of inhibition zone of antagonistic bacteria strain was used as a rule of evaluating the antagonistic performance.Some single factor experiments including of carbon sources,nitrogen sources and minerals and orthogonal experiments of optimal fermentation medium and fermentation conditions were respectively carried out in this study.The results showed that the optimal fermentation medium composition was：3g/l soluble starch,12g/L peptone and 3g/Lcalcium chloridize and using this optimal fermentation to cultivate this antagonistic bacteria under the condition of initial pH 8.0,8% inoculum and fermentative medium volume of 75 mL per 250 mL flasks for 24 h,the antagonistic bacterial showed the highest antagonistic capacity with about 2.6 cm of inhibition zone.All these results will give some references for further enhancing the ability of antagonistic bacteria and also offer some theoretical premises for better utilization of this antagonistic bacteria. |
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| Keywords: | antagonistic bacteria inhibition zone optimization of fermentative medium |
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