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| H2S对兔左心室心肌细胞电生理特性的影响 | |
| 引用本文： | 陈立锋,马淑红,范凌,赵兰平,温晓竞,马建伟,张晓云,王新满.H2S对兔左心室心肌细胞电生理特性的影响[J].河北北方学院学报(医学版),2009,26(2). |
| 作者姓名： | 陈立锋 马淑红 范凌 赵兰平 温晓竞 马建伟 张晓云 王新满 |
| 作者单位： | 1. 河北北方学院生理教研室,河北,张家口,075000 2. 河北省石家庄市灵寿县医院,河北,灵寿,050500 3. 河北北方学院附属第一医院血液科,河北,张家口,075000 4. 河北北方学院麻醉系2006级1班,河北,张家口,075000 |
| 基金项目： | 河北省教育厅自然科学研究项目，河北北方学院校基金 |
| 摘 要： | 目的:探讨硫化氢(H2S)对兔左心室心肌细胞电生理特性的影响.方法:用标准微电极细胞内记录技术,观测硫化氢对兔左心室心肌细胞电生理特性的影响.观测指标:静息电位(resting potential,RP)、超射值(over-shoot,OS)、动作电位幅值(amplitude of action po-tential,APA)、0相最大除极速率(maximal rate of depolarization,Vmax)、复极20%、50%和90%时间(20%、50%and 90% of duration of action potential,APD20、APD50and APD90)及复极化平均速度(average rate of repolarization).结果:(1)用50μmol/L NaHS 灌流时,和正常对照组相比,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).(2)100μmol/L NaHS与对照组比较,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).和50μmol/L NaHS相比APD50缩短(P＜0.01)、APD90缩短(P＜0.05)、复极化平均速度增快(P<0.05).(3)200μmol/L NaHS、400μmol/L、1 000μmol/L NaHS可浓度依赖性的使 APA、RP、OS减小(P<0.01),APD20、APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.01).结论:H2S可浓度依赖性的影响兔左心室心肌细胞电生理特性. |
| 关 键 词： | 电生理学 硫化氢 肌细胞/心脏 |
Effects of Hydrogen Sulfide on Electrophysiological Features of Rabbit Ventricular Myocytes |
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| CHEN Li-feng,MA Shu-hong,FAN Ling,ZHAO Lan-ping,WEN Xiao-jing,MA Jian-wei,ZHANG Xiao-yun,WANG Xin-man.Effects of Hydrogen Sulfide on Electrophysiological Features of Rabbit Ventricular Myocytes[J].Journal of Hebei North University:Medical Edition,2009,26(2). | |
| Authors: | CHEN Li-feng MA Shu-hong FAN Ling ZHAO Lan-ping WEN Xiao-jing MA Jian-wei ZHANG Xiao-yun WANG Xin-man |
| Abstract: | Objective:To investigate the effects of hydrogen sulfide(H2S)on action potentials(Ap)of rabbit left ventricular myocytes.Methods:By using standard intracellular microelectrode technique to record Ap,hydrogen sulfide was used to investigate the electrophysiological features of left ventricular myocytes.Electrophysiological parameters examined were:RP(resting potential),APA(amplitude of action potential),Vmax(maximal rate of depolarization),OS(over-shoot),APD20,APD50 and APD90(20%,50% and 90% of duration of action potential)and average rate of repolarization.Results:(1)Perfusion with NaHS(H2S donor,50μmol/L)led to a significant decrease in APD50(P＜0.01),APD90(P＜0.01)and a significant increase in average rate of repolarization(P<0.05).(2)Application of 100μmol/L NaHS resulted in a significant shortening in APD50(P＜0.01),APD90(P＜0.01)and a significant increase in average rate of repolarization(P<0.05).Compared with the result of 50μmol/L NaHS,100μmol/L NaHS led to a significant shortening in APD50(P＜0.01),APD90(P＜0.05)and a significant increase appeared in average rate of repolarization(P<0.05).(3)Application of 200μmol/L,400μmol/L and 1 000μmol/L NaHS led to a significant decrease in APA,RP and OS,a significant shortening in APD20,APD50 and APD90,a significant increase in average rate of repolarization in a concentration-depedent manner.Conclusion:H2S could significantly affect the electrophysiology of guinea pig ventricular myocytes in a concentration-depedent manner. |
| Keywords: | Electrophysiology Hydrogen Sulfide Myocytes/Cardiac |
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