| 海藻酸钠固定化菌株JY04降解苯酚特性研究 |
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| 引用本文： | 冯俊,姜立春,王静静,李锦.海藻酸钠固定化菌株JY04降解苯酚特性研究[J].绵阳师范学院学报,2014(2):87-91. |
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| 作者姓名： | 冯俊 姜立春 王静静 李锦 |
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| 作者单位： | 绵阳师范学院分子生物学与生物制药重点实验室,四川绵阳621000 |
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| 基金项目： | 四川省教育厅项目（12ZB258,092B142）;绵阳师范学院项目（MA2009016,Mnu-JY1124）. |
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| 摘 要： | 以苯酚作为唯一碳源和能源的无机盐培养液对从绝缘材料厂排放的活性污泥进行驯化,从中分离筛选到1株苯酚降解菌,编号为JY04.采用正交实验设计方法优化了该菌株固定化条件,并且考察了该固定化细胞降解苯酚的最适条件.结果表明:在固定化条件为海藻酸钠(SA)浓度为5%,湿菌重/包埋剂为6/30,CaCl2浓度为3%时,固定化时间为8 h,降解率可达82.6%,并具有较好的机械强度.确定其对苯酚降解最适条件为pH 7.0,苯酚浓度为400 mg/L,温度32℃,接种量为25 g,培养时间42 h,其苯酚的降解率可达97.3%.该菌株固定化细胞的降解苯酚能力和耐受苯酚能力均大于游离细胞.固定化菌株对苯酚有较强的降解和耐受能力,是处理苯酚废水的良好菌种资源.
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| 关 键 词： | 海藻酸钠 苯酚 固定化 降解特性 |
On Characteristics of Degrading Phenol with Immobilized Strain JY04 by Sodium-aliginate |
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| FENG Jun,JIANG Li -chun,WANG Jing -jing,LI Jin.On Characteristics of Degrading Phenol with Immobilized Strain JY04 by Sodium-aliginate[J].Journal of Mianyang Normal University,2014(2):87-91. |
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| Authors: | FENG Jun JIANG Li -chun WANG Jing -jing LI Jin |
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| Institution: | (Key Laboratories for Molecular Biology and Biopharmaceuticals, Mianyang Normal University, Mianyang, Sichuan 621000) |
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| Abstract: | A phenol degradation bacterium （JY04） which was able to grow on phenol as sole carbon and energy sources was isolated from the activated sludge of the insulating materials factory as the source of bacteria. Orthogonal experiment design was adopted to ascertain the optimal conditions of the immobilization of Strain JY04 and studied the optimal conditions of the immobilized strain to degrade phenol. bacteria embedded ratio 1.0 ： 5.0 and then treated with 3.0 % CaCl2 for When SA concentration was 5 8 h, the highest degradation rate reached 82.6 % while the strength of the immobilized cell remained good enough. The efficient biodegradation of phenol occurred when the strain was cultured in the medium （ pH 7.0） containing 0.4 g/L phenol under 32 ℃ and 25 g inoculation quantum where the highest degradation rate reached 97.3 % after 42 h. The phenol degradation ability and resistance of the immobilized strain cells against phenol were both higher than the free cells. The immobilized cell is a valid strain that can be used to treat phenol wastewater at high concentration. |
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| Keywords: | Sodium alginate phenol immobilized degradation characteristics |
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