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| 四种药用蕨类DNA提取的比较研究 | |
| 引用本文： | 李先进,彭正松,张正英,阮期平.四种药用蕨类DNA提取的比较研究[J].绵阳师范学院学报,2008,27(8). |
| 作者姓名： | 李先进 彭正松 张正英 阮期平 |
| 作者单位： | 1. 西华师范大学生命科学学院,四川南充,637002;绵阳师范学院分子生物与生物制药重点实验室,四川,绵阳,621000 2. 西华师范大学生命科学学院,四川南充,637002 3. 绵阳师范学院分子生物与生物制药重点实验室,四川,绵阳,621000 |
| 摘 要： | 以四种药用蕨类的叶片为材料，采用改进的CTAB法、SDS法分别对四种蕨类进行了总DNA提取效果的比较分析，结果表明，通过实验获得最佳的DNA提取方法，为PCR扩增，引物设计，为筛选四种药用蕨类中的活性成份的相关基因奠定理论和实践基础。两种方法均能有效的提取较高质量的DNA，其中CTAB法效果最好，获得的DNA纯度较高、产量较大；SDS法提取的量次之且多糖等杂质含量更多。 |
| 关 键 词： | 蕨类植物 新鲜叶片 DNA提取 SDS法 CTAB法 |
A Study of Different Methods for DNA Extraction from Pteridophyte |
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| LI Xian-jin,PENG Zheng-song,ZHANG Zheng-ying,RUAN Qi-ping.A Study of Different Methods for DNA Extraction from Pteridophyte[J].Journal of Mianyang Normal University,2008,27(8). | |
| Authors: | LI Xian-jin PENG Zheng-song ZHANG Zheng-ying RUAN Qi-ping |
| Abstract: | The total DNA has been extracted from the fresh leaves of 4 medicinal species of pteridophyte with CTAB,SDS,to compare their effects on DNA extraction.The result shows that the optimum preparation method obtained to extract high-quality DNA lays theoretic and practical base for PCR amplification and primer design,for filtrating related gene of the active elements from leaves of the four pteridophytes.Although high-quality DNA can be obtained by using both methods,the effect of CTAB is better than that of SDS. |
| Keywords: | pteridophyte fresh leaves DNA extraction SDS CTAB |
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